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ENDURO Horizontal Electrophoresis Systems

This guide details the step-by-step procedure for using Enduro Horizontal Electrophoresis Systems. It covers essential tasks including casting a gel, running the gel, and staining the gel for analysis. You'll learn how to properly prepare agarose solutions, attach buffer dams, and use the gel box effectively. The system features durable molded chambers, removable electrodes, and multichannel pipette-compatible combs, ensuring reliability and ease of use. Follow this guide for efficient gel electrophoresis and optimal results in your laboratory work.

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ENDURO Horizontal Electrophoresis Systems

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  1. ENDURO Horizontal Electrophoresis Systems

  2. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of gel tray Place gel tray on a flat surface

  3. Casting a gel • Mix agarose buffer solution and boil • Cool agarose solution to below 60ºC • Pour onto gel tray • Let solution cool for ½ hour • Gently remove combs

  4. Running the Gel • Remove the casting gates • Place the gel tray into the gel box • Overlay it with buffer • Connect the lid & attach to a power supply

  5. Staining the Gel • Remove gel from gel tray • Place into a container with stain • Destain the gel with buffer or water

  6. Features • Heavy duty molded chambers • Removable electrodes • Multichannel pipette compatible combs • Domed lid to prevent condensation

  7. Heavy duty molded construction • Prevents leaking problems

  8. Changing the electrode Turn unit over & remove screw Remove old electrode Insert new electrode & secure with nylon screw

  9. Multichannel Combs A large number of comb are compatible with multichannel pipettes

  10. Domed Lid Prevents condensation from falling on the gel Recaptures condensation in reservoirs

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