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Comprehensive Guide to Enduro Horizontal Electrophoresis Systems Use and Setup

This guide provides a step-by-step overview of using the Enduro Horizontal Electrophoresis System, covering the gel casting, running, and staining processes. Learn how to properly set up your gel tray with buffer dams and mix your agarose solution for optimal results. Discover features like heavy-duty molded chambers and removable electrodes that enhance usability. The guide also explains electrode changing and highlights compatibility with multichannel pipettes, ensuring efficient lab experiments. Ideal for researchers and students in molecular biology.

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Comprehensive Guide to Enduro Horizontal Electrophoresis Systems Use and Setup

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  1. ENDURO Horizontal Electrophoresis Systems

  2. Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of gel tray Place gel tray on a flat surface

  3. Casting a gel • Mix agarose buffer solution and boil • Cool agarose solution to below 60ºC • Pour onto gel tray • Let solution cool for ½ hour • Gently remove combs

  4. Running the Gel • Remove the casting gates • Place the gel tray into the gel box • Overlay it with buffer • Connect the lid & attach to a power supply

  5. Staining the Gel • Remove gel from gel tray • Place into a container with stain • Destain the gel with buffer or water

  6. Features • Heavy duty molded chambers • Removable electrodes • Multichannel pipette compatible combs • Domed lid to prevent condensation

  7. Heavy duty molded construction • Prevents leaking problems

  8. Changing the electrode Turn unit over & remove screw Remove old electrode Insert new electrode & secure with nylon screw

  9. Multichannel Combs A large number of comb are compatible with multichannel pipettes

  10. Domed Lid Prevents condensation from falling on the gel Recaptures condensation in reservoirs

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