CaFbFP AGATCTATGGC T TC A TT T CA A TC A TT T GG T AT T CC A GG T CA AT TGGAAGT

1. BglII CaFbFP AGATCTATGGCTTCATTTCAATCATTTGGTATTCCAGGTCAATTGGAAGT EcFbFP ------ATGGCGTCGTTCCAGTCGTTCGGCATCCCGGGCCAGCTGGAAGT M A S F Q S F G I P G Q L E V CaFbFP TATTAAAAAAGCTTTGGATCATGTTAGAGTTGGTGTTGTTATTACTGATC EcFbFP CATCAAGAAGGCGCTGGATCACGTGCGCGTCGGCGTGGTCATCACCGATC I K K A L D H V R V G V V I T D P CaFbFP CAGCTTTGGAAGATAATCCAATTGTTTATGTTAATCAAGGTTTTGTTCAA EcFbFP CCGCGCTGGAAGATAACCCGATCGTCTACGTGAACCAGGGCTTCGTGCAG A L E D N P I V Y V N Q G F V Q CaFbFP ATGACTGGTTATGAAACTGAAGAAATTTTGGGTAAAAATGCTAGATTTTT EcFbFP ATGACCGGCTACGAGACCGAGGAAATCCTGGGCAAGAACGCGCGCTTCCT M T G Y E T E E I L G K N A R F L CaFbFP GCAAGGTAAACATACTGATCCAGCTGAAGTTGATAATATTAGAACTGCTT EcFbFP CCAGGGGAAGCACACCGATCCGGCGGAAGTGGACAACATCCGCACCGCGC Q G K H T D P A E V D N I R T A L CaFbFP TGCAAAATAAAGAACCAGTTACTGTTCAAATTCAAAATTATAAAAAAGAT EcFbFP TGCAAAATAAAGAACCGGTCACCGTGCAGATCCAGAACTACAAGAAGGAC Q N K E P V T V Q I Q N Y K K D CaFbFP GGTACTATGTTTTGGAATGAATTGAATATTGATCCAATGGAAATTGAAGA EcFbFP GGCACGATGTTCTGGAACGAACTGAACATCGATCCGATGGAAATCGAGGA G T M F W N E L N I D P M E I E D CaFbFP TAAAACTTATTTTGTTGGTATTCAAAATGATATTACTAAACAAAAAGAAT EcFbFP TAAGACGTATTTCGTCGGCATCCAGAACGACATCACCAAGCAGAAGGAAT K T Y F V G I Q N D I T K Q K E Y CaFbFP ATGAAAAATTGTTGGAATAATAGGGATCC EcFbFP ATGAAAAGCTGCTCGAGTGA--------- E K L L E * HindIII AvaI BamHI CTCGGG Supplemental figure. Alignment of the EcFbFP and CaFbFP expressing genes. The nucleotide sequence of EcFbFP gene was modified according to the codon usage of C. albicans and an additional stop codon was added. For cloning of CaFbFP into pDS1044 BglII and BamHI sites were generated, which were replaced by BamHI and EcoRI sites for the construction of pIE3. As indicated, in order to produce a fusion of two CaFbFP genes an assymetrical AvaI site was inserted preceding the first stop codon.