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Gene Expression Analysis in U937 Human Immune Cells Treated with Estrogen and 4-Nonylphenol

This study utilized the methodology of treating U937 human immune cells with estrogen and 4-nonylphenol at specific concentrations. RNA isolation and RT to cDNA processes were performed before applying cDNA to microarray chips and analyzing the data using Spotfire software. RT-PCR was conducted to measure the expression of ER-beta, Bcl-2, and Her-2 genes, with B-actin serving as the reference gene. Gel electrophoresis on a 2% agarose gel was employed for further analysis.

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Gene Expression Analysis in U937 Human Immune Cells Treated with Estrogen and 4-Nonylphenol

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  1. Methodology U937 Human Immune Cells Estrogen (5 uM) Control (No treatment) 4-nonylphenol (5 uM) Cultured Cells, RNA Isolation, RT to cDNA Applied cDNA to microarray chips Scanned chips for detection of gene expression by chemiluminescence Data analyzed by Spotfire software RT-PCR (using ER-beta, Bcl-2, Her-2) Reference gene: B-actin 2% agarose gel electrophoresis

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