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A. Raw 264.7 cell

← MMP-9. ← MMP-2. Data supplement 3. Transient treatment with decursin do not block LPS induced activation of MMP-9 in Raw264.7 cells and THP-1 cells. A. Raw 264.7 cell. - - 20 40 60 80 100 - - -

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A. Raw 264.7 cell

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  1. ←MMP-9 ←MMP-2 Data supplement 3. Transient treatment with decursin do not block LPS induced activation of MMP-9 in Raw264.7 cells and THP-1 cells A. Raw 264.7 cell - - 20 40 60 80 100 - - - - - - - - - - 0.06 0.08 0.1 - + + + + + + + + + - - 20 40 60 80 100 - - - - - - - - - - 0.06 0.08 0.1 - + + + + + + + + + Decursin (μM) EtOH (%) LPS 100 ng/ml No washing Washing B. THP-1 cell - - 20 40 60 80 100 - - - - - - - - - - 0.06 0.08 0.1 - + + + + + + + + + - - 20 40 60 80 100 - - - - - - - - - - 0.06 0.08 0.1 - + + + + + + + + + Decursin (μM) EtOH (%) LPS 1 μg/ml ←MMP-9 ←MMP-2 No washing Washing RAW264.7 (A) or THP-1 cells (B) were pretreated with indicated concentrations of decursin for 2 hrs. Half of the cells were stimulated with 1 mg/ml LPS while the other half of the cells were washed three times to remove decursin and then stimulated with same amount of LPS. Culture supernatants were collected in 24 hours and MMP-9 levels were measured using gelatin zymogram.

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