ANNA UNIVERSITY CENTRE FOR BIOTECHNOLOGY. Screening of biological properties of Medicinal plants for anticancer Activity using In vitro techniques - An approach towards Anticancer Drug Development. Senthil.V. *, Jis.C.Joy, Keerthivasan.A, Giridharan.P and Balakrishnan.A
CENTRE FOR BIOTECHNOLOGY
Screening of biological properties of Medicinal plants for anticancer Activity using In vitro techniques - An approach towards Anticancer Drug Development.
Senthil.V.*, Jis.C.Joy, Keerthivasan.A, Giridharan.P and Balakrishnan.A
Centre for Biotechnology, Anna University, Chennai-600 025, India.
Medicinal plants are the most exclusive source of life saving drugs for the majority of the world’s population. Medicinal herbs have been widely used for treatment of diseases in traditional way for several generations. An interaction between traditional medicine and modern biotechnological tools is to be established towards New Drug development. The interface between cell biology, in vitro assays and structural chemistry will be the best way forward to obtain valuable leads.
Preliminary - Thymidine Incorporation assay
Confirmation of mechanism using analysis of apoptotic markers
Confirmation of apoptosis
MAJOR APOPTOTIC PATHWAYS
Death Receptor Pathway
Structure of Andrographis paniculata pure compound
Ref: Balmain.A , et al, J.Chem.Soc, Perkin Trans 1,1973,1247
Thymidine incorporation studies showed a significant anti-proliferative activity of the Crude and Pure compounds isolated from Andrographis paniculata at a dose concentration of Crude 25µg/ml and Pure 12.5 µg/ml at a time point of 24 and 48 hours
TRANSCRIPTIONAL EXPRESSION PROFILE
RT-PCR studies reveal the activation of p53 followed by the activation of bax and downregulation of bcl-2 in HeLa cells treated with Crude extract and Pure compound of the Andrographis paniculata at a time point of 12 hours.
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TRANSLATIONAL EXPRESSION PROFILE
The western blot analysis confirmed the down regulation of the anti-apoptotic protein Bcl-2 leading to Cytochrome c release and activation of Caspase 3.
A. paniculata Crude extract
A. paniculata Pure extract
DNA FRAGMENTATION ASSAY
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Apoptosis is confirmed by DNA fragmentation, observed in cells treated with crude extract and pure compound of Andrographis paniculata
Suppressed Bcl-2 expression
Up-regulation of pro-apoptotic protein Bax
Cytochrome c release from mitochondria
Activation of Caspase-3
MECHANISM OF ACTION