Cost is prohibitive to assess all NMs Published ES&T Feb 2009 Phase 1 Screen 20 NP’s identified via High-Throughput system (HTS) at UCLA Phase 2 5-10 NP’s identified in phase 1 Phase 3 2-3 NP’s from phase 2 IRG2 : Interactions at Molecular, Cellular, Organ & Systemic Levels
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Screen 20 NP’s identified via High-Throughput system (HTS) at UCLA
5-10 NP’s identified in phase 1
2-3 NP’s from phase 2
IRG2: Interactions at Molecular, Cellular,
Organ & Systemic Levels
Trophic Transfer & Biomagnification of CdSe Quantum Dots
High Throughput Screening and Data Mining based on
property-activity relationships that can be used to rank NM for
risk and priority in vivo testing
UC CEIN: Predictive Toxicology Assessment and Safe Implementation of Nanotechnology in the Environment
Modeling Regulatory Challenges in NM Lifecycle
US Public Environmental RP Survey
A. Nel1,2,3,4,Y. Cohen2,3,5, H. Godwin1,2,3, A. Keller2,6, R. Nisbet2,7
IRGs 5, 6, 7
780-fold QD Biomagnification
5.6-fold QD Bioaccumulation
High Throughput Bacterial,
Cellular or Molecular Screening
Mission and Objectives
Prioritize in vivo testing
at increasing trophic levels
UC CEIN Interdisciplinary Research Groups (IRGs)
IRG5: High Throughput Screening to Develop Predictive Toxicological Paradigms based on Material Properties
IRG6: Develop Decision Tool to Assess the Environmental Impact of Nanomaterials
IRG1: Standard Reference and Combinatorial Libraries
IRG7: Environmental Risk Perception
Combinatorial library designed to provide the same material in different sizes, shapes, roughness, aspect ratios, states of dispersal, chemical composition, etc.
TiO2, CeO2, ZnO, and NH2PS
Data collection in progress
25 mL NP suspension at 50, 25, 12.5, 6.25 and 3.125 mg/mL
Aug 2008 ASA presentation
25 mL of dye
EST 2009 publication
Nature Nano 2009; ChemE 2009 (in press)
NM libraries &
IRG Leader – Barbara Herr Harthorn, UCSB
High Throughput Screening
Computerized expert system, multimedia
modeling, risk ranking
Organism, population, community & ecosystem toxicology
Molecular, cellular, & organ injury pathways
Fate & Transport
R. Werlin, J.H. Priester, R.E. Mielke, S. Jackson, G.D. Stucky, G. Cherr, E. Orias, P.A. Holden
IRG Leader – Jeffrey Zink, UCLA
IRG Leader – Patricia Holden, UCSB
IRG Leader – Hunter Lenihan, UCSB
Challenges: Screening the effects of new nanomaterials (NM) requires the development of models for the environmental distribution of NM and their toxicity.
IRG3: Effects of Nanomaterials on Marine Ecosystems
IRG4: Nanoparticle Fate and Transport
Phase 1: Rapid Toxicity Bioassays
Phase 2: Toxic effects predicted by IRG2 (ROS, lysosomal stability, apoptosis)
IRG4 research focuses on understanding the mobility and bioavailability of NPs in different environmental conditions. Our work with metal oxide NPs has shown that they can be easily stabilized under freshwater conditions, which is a major pathway from the sources (e.g. wastewater treatment plant discharge, stormwater, other runoff) into other environmental compartments, such as estuaries and oceans, where the particles sediment rapidly. This has important implications for aquatic organisms that are exposed to particles either in the water column or sediments.
Solutions: Develop and apply machine learning techniques for NM classification and property predictions; Apply multimedia transport and fate models to evaluate the dynamic mass distribution of NM, and Apply decision tools incorporating quantitative and qualitative information for decision making
Phase 3: Mesocosm experiments for species interactions, bioaccumulation and biomagnification
1 Department of Environmental Health Science, UCLA School of Public Health, 16-035 CHS, BOX 951772, Los Angeles, CA 90095.
2 UC Center for the Environmental Implications of Nanotechnology (UC CEIN), University of California Los Angeles, 6522 CNSI, 570 Westwood Plaza, Los Angeles, CA 90095-7277.
3 California NanoSystems Institute, University of California Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095.
4 Division of NanoMedicine, Department of Medicine, University of California, Los Angeles, California 90095.
5Department of Chemical and Biomolecular Engineering, 5531 Boelter Hall, University of California Los Angeles, Los Angeles, California, USA
6 School of Environmental Science and Management, University of California, Santa Barbara
7 Department of Ecology, Evolution & Marine Biology University of California Santa Barbara
IRG Leader – Yoram Cohen, UCLA
IRG Leader – Arturo Keller, UCSB
IRG Leader – Kenneth Bradley, UCLA
Coastal Marine Food Web
The UC Center for Environmental Implications of Nanotechnology (UC CEIN) at UC Los Angeles (UCLA) is in partnership with UC Santa Barbara (UCSB), UC Davis (UCD), UC Riverside (UCR), Columbia University in New York, the Molecular Foundry at Lawrence Berkeley National Laboratory (LBNL), the Lawrence Livermore National Laboratory (LLNL), Nanyang Technological University in Singapore (NTU), University of New Mexico (UNM), Sandia National Laboratory (SNL), University of Texas in El Paso (UTEP), University of Bremen (Germany), University of British Columbia (UBC), Cardiff University (Wales), University College Dublin (UCD, Ireland), and Universitat Rovira i Virgili in Spain (URV).
This material is based upon work supported by the National Science Foundation and the Environmental Protection Agency under Cooperative Agreement Number EF 0830117. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation or the Environmental Protection Agency. This work has not been subjected to EPA review and no official endorsement should be inferred.