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+. -. +. GST-Myc 1-262. -. +. -. GST. -. +. +. Abl. a. b. Flag-Myc 5YF. -. -. -. +. GST Myc 1-262. -. +. -. +. Flag-Myc WT. P-Tyr. Abl. -. -. +. +. Abl. P-Tyr. Total:. IP:Flag. Myc. GST Myc 1-262. GST. Total:. Abl. GST. c. d. -. -. -. -. +. Myc T58A.

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  1. + - + GST-Myc 1-262 - + - GST - + + Abl a b Flag-Myc 5YF - - - + GST Myc 1-262 - + - + Flag-Myc WT P-Tyr Abl - - + + Abl P-Tyr Total: IP:Flag Myc GST Myc 1-262 GST Total: Abl GST c d - - - - + Myc T58A - - + + Bcr-Abl - - - + - Myc 5YF - - - + Flag-Myc Y74F Myc WT - - - + - + + - + Flag-Myc WT Bcr-Abl - - + + + P-Tyr IP Myc pY74 Flag Myc Total Flag Bcr-Abl Total Vinculin Bcr-Abl Sanchez-Arévalo et al. Fig. S2

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