1 / 35

Automated High Content Imaging and Data Processing for Yeast Analysis

Explore a comprehensive guide on high content imaging techniques for yeast analysis with a focus on reproducibility, segmentation, and automation. Learn how to acquire and analyze data efficiently. Utilize VBA code to group, classify, and compare colonies between control and experimental plates. Take advantage of tools like the Yeast Robot, flatbed scanner, and Axiovision for automated segmentation and data processing.

owen-holland
Download Presentation

Automated High Content Imaging and Data Processing for Yeast Analysis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Higher Content Yeast Imaging and Data Processing Richard Alexander Stowers Microscopy Center May 19, 2009

  2. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  3. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  4. Control Plate Experimental Plate

  5. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  6. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  7. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  8. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  9. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  10. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  11. Use VBA Code to… • Group Colonies • Classify Colonies • Compare between control and experimental plates

  12. Use VBA Code to… • Group Colonies • Classify Colonies • Compare between control and experimental plates

  13. A B C • Use VBA Code to… • Group Colonies • Classify Colonies • Compare between control and experimental plates 1 2 3

  14. Summary • Yeast Robot • Pin yeast plate • Flatbed Scanner • Image yeast plate • Axiovision • Automate Segmentation • Automate Data Acquisition • Excel • Automate Data Processing

  15. Acknowledgements • Rong Li • Das Arupratan • Brian Slaughter • Molecular Biology • Dan Bradford • Brian Sanderson

More Related