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Quantitative Assessment of Mesozooplankton Biomass and Production

Comprehensive study to quantify mesozooplankton biomass, impacts on phytoplankton stocks, and matter flux. Combining innovative and classical approaches for detailed analysis of zooplankton communities. Sampling, processing, and measurement methods outlined for accurate data collection and analysis.

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Quantitative Assessment of Mesozooplankton Biomass and Production

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  1. Mesozooplankton component • General objective: Quantitative assessment of its biomass and production and its impacts on phytoplankton stocks and vertical matter flux. • Approaches: • 1. The biomass (as organic carbon and nitrogen) determined by combination on size structure (estimates based on OPC/ZOOSCAN measurements)* and direct determination of carbon and nitrogen contents(with CHN)* in mesozooplankton fractions. • 2. The samples for description of zooplankton communities*. • 3. Complementary process studies to estimate biological rates (details to be discussed during this meeting **) and fluxes through the compartment. • - Egg production experiments* • - Others • *: Innovative approach • *: Classical approach

  2. Details ►Sampling 1. - Bongo net tows for samples Standard: Depth from 200 m to the surface Alternatives: - Depth defined in real time observation from size particles distribution UVP (to be discussed). - Open-close nets 2. - Net tows for living organisms

  3. ►Processing on board: • 1. Collected samples • Conditioning samples for preservation • Conditioning samples for weighting C/N: filtrations • 2. Collected living organisms • 24 h Egg production experiments

  4. ►Measurement Lab OPC/ZOOSCAN: Fine resolution if the size-structured biomass Binocular: Faunistic distributions Counting from egg experiments

  5. ►Objectives : 1. Quantify mesozooplankton biomass (200 µm-2000 µm) based on a fine resolution of its size structure using OPC and ZOOSCAN, and CHN weighting. Deliverables -         Total biomass -         Size-fraction biomasses (200-300 µm;300-500 µm;500-1000 µm; >1000 µm) from OPC/ZOOSCAN and from CHN on similar size fraction -         Fine size-distribution of biomass from OPC and binocular: define main contributors

  6. ►Objectives (suite): 2. Description of zooplankton communities Deliverables -         Taxonomic tables per station -         Identification of main contributors on size spectra 3. Derived index of zooplankton production with the slope of the biomass size spectrum Deliverables -         Index of production -         Derived fluxes as allometric relationship to weight: Respiration of planktonic communities by the use of biomass spectra and allometric relations

  7. ►Objectives (suite): 4. Estimate zooplankton production from classical method: egg production of copepods Deliverable: Egg production per female Egg production per biomass fraction – Secondary production

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