1 / 20

PREPARATION OF A BACTERIAL SMEAR SPECIAL STAINING TECHNIQUES

PREPARATION OF A BACTERIAL SMEAR SPECIAL STAINING TECHNIQUES. ASEPTIC PROCECURE FOR MICROBE REMOVAL. http://www.youtube.com/watch?v=0odxJy0nR9s&feature=related. ASEPTIC PROCECURE FOR MICROBE REMOVAL FOR PREPARATION OF A BACTERIAL SMEAR.

oprah
Download Presentation

PREPARATION OF A BACTERIAL SMEAR SPECIAL STAINING TECHNIQUES

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. PREPARATION OF A BACTERIAL SMEARSPECIAL STAINING TECHNIQUES

  2. ASEPTIC PROCECURE FOR MICROBE REMOVAL http://www.youtube.com/watch?v=0odxJy0nR9s&feature=related

  3. ASEPTIC PROCECURE FOR MICROBE REMOVAL FOR PREPARATION OF A BACTERIAL SMEAR

  4. http://www.youtube.com/watch?v=HxjN2mBLGCE&feature=related http://www.youtube.com/watch?v=n-HHI4E1LhI&feature=related

  5. SPECIMEN PREPARATION FOR LIGHT MICROSCOPY • PREPARATION OF A BACTERIAL SMEAR

  6. STAINING • SPECIAL STAINING TECHNIQUES • Negative Staining for Capsules • Capsules • Determinants for virulence in some genera of bacteria • Capsule Staining • Preparation of a bacterial smear along with negative staining w/ Nigrosin or India Ink • Counterstain w/ Crystal Violet

  7. STAINING • SPECIAL STAINING TECHNIQUES • Negative Staining for Capsules • Preparing a bacterial smear for Capsule Staining

  8. STAINING • SPECIAL STAINING TECHNIQUES • Negative Staining for Capsules • Capsule Stain Results

  9. STAINING • SPECIAL STAINING TECHNIQUES • Endospore Stain • Genera of bacteria that produce endospores • Bacillus & Clostridium • Other genera: Desulfotomaculum, Sporosarcina, Sporolactobacillus, Oscillospira,Thermoactinomyces • Schaeffer-Fulton Method for Endospore Staining • Preparation of a Bacterial Smear-Air dry • Application of Primary Dye (Malachite Green) w/ concurrent application of heat • Allow slide to cool • Rinse w/ water • Application of Counterstain (Safranin)

  10. http://www.tgw1916.net/video_pages/Spore.html http://www.answers.com/topic/endospore

  11. STAINING http://www.tgw1916.net/movies.html • SPECIAL STAINING TECHNIQUES • Endospore Stain Results • Flagellar Stain • Uses mordant and carbolfuchsin stain to increase flagellar diameter until resolved by light microscope http://www.answers.com/topic/endospore

More Related