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A. B. 1400. a. a. 1200. b. 1000. c. IL-6 (pg/ ml). 800. 600. 400. d. e. 200. 0. DMSO. 25. 50. 100. 200. 400. Minocycline ( m g/ml). C. 300. MINO. DMSO. 200. IL-1 b (pg/ ml). 100. 0. Saline. LPS. 2500. d. D. 2000. c. 1500. LPS. Saline. b. BV-2 mRNA

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slide1

A.

B.

1400

a

a

1200

b

1000

c

IL-6 (pg/ ml)

800

600

400

d

e

200

0

DMSO

25

50

100

200

400

Minocycline (mg/ml)

C.

300

MINO

DMSO

200

IL-1b (pg/ ml)

100

0

Saline

LPS

2500

d

D.

2000

c

1500

LPS

Saline

b

BV-2 mRNA

(fold change)

IL-6 (pg/ ml)

1000

DMSO

MINO

DMSO

MINO

MHC II

3.86 ± 0.41b

0.80 ±0.10a

2.34 ± 0.22c

1.0 ± 0.09a

a

500

a

a

TLR-2

1.0 ± 0.08a

0.85 ± 0.09a

4.58 ± 0.80b

3.23 ± 0.15c

0

IL-1b

1.0 ± 0.12a

1.0 ± 0.20a

223.7 ± 17.9b

132.3 ± 17.1c

0

0.1

1

10

100

1000

IL-6

1.0 ± 0.07a

1.9 ± 0.16a

418.8 ± 55.6b

230.2 ± 25.0c

LPS (ng/ml)