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Genotypic and Phenotypic Characterization of C. neoformans var. grubii Strains

This study examines multiple strains of Cryptococcus neoformans var. grubii, focusing on their genotypes and phenotypic characteristics. The strains include H99, F99, KN99a, and others sourced from various studies. All strains exhibit MATa mating type with specific mutations in genes such as gpa1, pka1, crg2, and ADE2. A comprehensive analysis of oligonucleotide primers used for amplification in this research is also provided, facilitating a deeper understanding of the genetic and functional diversity within C. neoformans populations.

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Genotypic and Phenotypic Characterization of C. neoformans var. grubii Strains

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  1. Table 1. C. neoformans var. grubii strains used in this study Genotype Strains Source H99 F99 KN99a F99a AAC1 CDC1 PWC279 PWC201 PWC572 PWC111 PWC195 PWC201 PWC279 PWC324 PWC403 PWC424 PWC427 PWC429 PWC434 PWC435 PWC478 Perfect, et al., 1993 Wang, et al., 2002 Nielsen, et al., 2003 Fraser & Heitman Alspaugh, et al., 1997 D’Souza, et al., 2000 Li, et al., 2007 Li, et al., 2007 Li, et al., 2007 Davidson, et al., 2004 This study This study This study This study This study This study This study This study This study This study This study MATa MATa ura5 MATa ura5 MATa ura5 MATa gpa1::ADE2 MATa pka1::ADE2 MATa gpa2::URA5 MATa gpa3::URA5 MATa gpa2::URA5 gpa3::URA5 MATa cpk1::URA5 MATa crg2::URA5 MATa crg2::NAT1 MATa crg2::URA5 CRG2::NAT1 MATa crg2::NAT1 gpa2::URA5 MATa crg2::NAT1 gpa3::URA5 MATa crg2::NAT1 gpa2::URA5 gpa3::URA5 MATa crg2::NAT1 gpa1::URA5 MATa crg2::NAT1 pka1::URA5 MATa crg2::NAT1 cpk1::URA5 MATa gpa1::Ade2 GPA1Q284L::NAT1 MATa crg2::NAT1 gpa1::ADE2 GPA1Q284L::NAT1 Supplemental Table I Shen et al.

  2. Table 2. Oligonucleotide primers used in this study Primer Sequence PW160 5’-GAAGATCTGCGACTAAAGCAGATCCTCGAG-3’ PW181 5’-AACTGCAGCTAGTCTACAGTCTGTGACCGG-3’ PW206 5’-GAATTCACCTTTCTTCGCCGTCCAGATACT-3’ PW227 5’-CCAGCAGATAAGTTTGAACCCGGGTAAGAGGAAAAGCAAA-3’ PW228 5’-TTTGCTTTTCCTCTTACCCGGGTTCAAACTTATCTGCTGG-3’ PW270 5’-AACTGCAGTGCCGATTGAAATAACTCGCC-3’ PW273 5’-AACTGCAGATGGCGGTTTTCCTGTTGAGG-3’ PW290 5’-AAGAATTCTTTCTCCGCAGACCTGACAAAA-3’ PW359 5’-AAGGATCCAAATGGCAGAAAGCAACGAGAAGA-3’ PW402 5’-GAGGATCCACCTTACAGGAGGTACTGGAC-3’ PW403 5’-GACTGCAGTTAAGTAAGATTGGCAAAGGC-3’ JH12499 5’-CATTCATATGTTCGATGTCGGTGGACTGAGAAGCGAGAGAAAGAAGTGG-3’ JH12500 5’-CCACTTCTTTCTCTCGCTTCTCAGTCCACCGACATCGAACATATGAATG-3’ Supplemental Table II Shen et al.

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