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Investigation of WA and TMZ Effects on U251, U138, and T98G Glioblastoma Cell Lines

This study explores the impact of varying concentrations of WA (0.5µM to 100µM) and TMZ (300µM) on the expression of key proteins in U251, U138, and T98G glioblastoma cell lines. We measured levels of HSP70, HSP32, AMPKα, p-AMPKα, mTOR, and p-mTOR, along with actin as a loading control. Results reveal the differential signaling responses across cell lines, enhancing our understanding of therapeutic mechanisms and resistance patterns in glioblastoma treatment.

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Investigation of WA and TMZ Effects on U251, U138, and T98G Glioblastoma Cell Lines

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  1. a U251 U87 ** ** ** b ___ ___ ___ ___ ___ ___ ___ ___ 0.5µM WA 0µM WA 1µM WA 2.5µM WA 5µM WA 0µM WA 1µM WA 2.5µM WA 100µM TMZ- + - - + - - + - - + - - + - - + - - + - - + - 300µM TMZ- - + - - + - - + - - + - - + - - + - - + - - + kDa HSP70 72 32 HSP32 AMPKα 62 p-AMPKα 62 200 mTOR p-mTOR 200 Actin 43 U251TMZ U138 HSP70 HSP32 AMPKα p-AMPKα mTOR p-mTOR Actin T98G HSP70 HSP32 AMPKα p-AMPKα mTOR p-mTOR Actin U138

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