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Use of infrared video thermography for study of freezing in transgenic and non-transgenic wheat

Use of infrared video thermography for study of freezing in transgenic and non-transgenic wheat. Joan Vickers 1 , Mick Fuller 2, Troy Fredericks 3 , Jack Christopher 3 , Mark Sutherland 1 and Grant Daggard 1.

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Use of infrared video thermography for study of freezing in transgenic and non-transgenic wheat

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  1. Use of infrared video thermography for study of freezing in transgenic and non-transgenic wheat Joan Vickers1, Mick Fuller2, Troy Fredericks3, Jack Christopher3, Mark Sutherland1 and Grant Daggard1 1 University of Southern Queensland, Centre for Systems Biology,Department of Biol/Phys Sciences, Toowoomba, QLD, Australia 2 School of Biological Sciences, University of Plymouth, UK 3 Leslie Research Centre, Queensland Department of Primary Industries & Fisheries, Toowoomba, QLD, Australia

  2. Delayed planting to avoid frost can result in significant yield penalties (approx 1% yield loss/day delay) Frost damage is a significant concern:2. Indirect losses Frost damage can be costly: (GRDC Advice Sheet to farmers, March 2006): • $100M/yrNorthern NSW and Queensland (direct frost damage) • $90M Western Australia (2004/5) • $33M/yr South Australia and Victoria.

  3. Radiation frost on wheat at ear emergence

  4. Infrared thermography

  5. Infrared video camera aimed at wheat plants in AGRF Adelaide frost chamber

  6. Wheat plants in AGRF frost chamber (-4.5C) • Only 10% of heads froze, remaining heads supercooled • No partial freezing of heads was observed • Suggests that FIS is not an effect of freezing, but is better described as a chilling injury Endotherms at thaw Camera view Freeze dehydration Exotherm (-4.5C)

  7. Conclusions – wheat and IVT • Infrared video thermography in wheat is useful to: • Visualise the process of freezing • Determine mechanisms of initiation and propagation of freezing exotherms • Compare thermodynamic events in different tissues • Demonstrate whether or not freezing has occurred • Monitor effect of modifications of field environment on wheat

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