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Schematic Representation of Transformation Constructs Carrying Chimeric Genes in Vector pS1301

This document presents a detailed schematic diagram of the transformation construct utilizing the pS1301 vector, which carries four chimeric genes: D52S, D56S, D57S, and D92S. The constructs include critical components such as RB (right T-DNA border), LB (left T-DNA border), and genes for β-glucuronidase (GUS) and hygromycin phosphotransferase (Hpt). Additionally, the cauliflower mosaic virus 35S promoter (P35S) and the 5’ nontranslated region of the tobacco etch virus (TEVL) are incorporated, along with the nopaline synthase (NOS) polyadenylation signal.

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Schematic Representation of Transformation Constructs Carrying Chimeric Genes in Vector pS1301

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  1. BamHI EcoRI LB Hpt P35S TEVL Chimeric gene NOS GUS RB Vector pS1301 carrying chimeric gene SupplementaryFigure 1. Schematic diagrams of the transformation construct. RB and LB, right and left T-DNA border; GUS, -glucuronidase gene; Hpt, hygromycin phosphotransferase gene; P35S, cauliflower mosaic virus 35S promoter; TEVL, 5‘-nontranslated region of the tobacco etch virus; NOS, nopaline synthase polyadenylation signal. Four chimeric genes, D52S, D56S, D57S, and D92S, were cloned into this vector.

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