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Overall Picture. Plasmid IsolationRemove plasmid pBS 60.6 from DHa E. coli. Restriction Enzymes. Each restriction enzyme cuts at different sites The cut can leave either blunt or sticky endsIf the plasmid and gene to be inserted into the plasmid are cut with the same restriction enzyme, the sticky ends have overlapping base pairs and can anneal with DNA ligaseBut, the cut ends can anneal to themselves or the cut vector can reanneal to itself Can prevent vector religation by using two restri21

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