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Serologi

Serologi. SEROLOGI FORENSIK Bahan: Darah, saliva, semen dan cairan tubuh lain (sekretor 80% dari populasi) Barang bukti penting kasus forensik Darah : - Cairan (plasma + serum )= 55% - Padatan (sel + fibrin)= 45% Darah: - air - sel - enzim, protein - substansi organik. antigen.

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Serologi

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  1. Serologi SEROLOGI FORENSIK Bahan: Darah, saliva, semen dan cairan tubuh lain (sekretor 80% dari populasi) Barang bukti penting kasus forensik Darah : - Cairan (plasma + serum )= 55% - Padatan (sel + fibrin)= 45% Darah: - air - sel - enzim, protein - substansi organik antigen

  2. Lanjutan • Plasma: • - air • - serum (kuning): - zat beku darah (fibrin) • Sel : • - sel darah merah (erytrocyt)-- penting untuk ahli forensik • - sel darah putih (leucocyte) -- Penting untuk imunolog • Sel darah merah: • - adanya antigen permukaan • - penting untuk uji forensik • Serum ---- mengandung antibodi-------- reaksi -- antigen><antibodi • Serologi forensik:---- barang bukti untuk kasus kriminal

  3. Golongan darah Golongan Antigen Antibodi darah (sel darah merah) (serum) _________________________________________________________________ A A anti-B B B anti-A AB AB bukan anti-A/anti-B O O anti-A/anti-B _________________________________________________________________

  4. Persentase jumlah populasi dan jumlah komponen rhesus (Rh) dalam setiap golongan darah O A B AB ------------------------------------------------------------------------------------------------------------ 43-45 % 40-42 % 10-12 % 3-5 % O+ 39% A+ 35% B+ 8% AB+ 4% O- 6% A- 5% B- 2% AB- 1% ---------------------------------------------------------------------------------------------------------- Golongan darah Rhesus satu dari jml-populasi ------------------------------------------------------------------------------------------------------------- O+ 1 3 O- 1 15 A+ 1 3 A- 1 16 B+ 1 12 B- 1 67 AB+ 1 29 AB- 1 167 ---------------------------------------------------------------------------------------------------------- Sub kelompok golongan darah: O1; O2 A1; A2 B1;B2 …….dsb

  5. Faktor Rhesus

  6. TRANSFUSI DARAH

  7. Clote / Penggumpalan darah

  8. Polymorfisme Bentuk enzim/protein polimorfisme: PGM2-1 Phosphoglucomutase EAP  Erythrocyt acid phosphatase EsD  Esterase-D AK  Adenyl – Kinase ADA  Adenosin deaminase GPT  Glutamic pyruvat transaminase 6-PGD  6-phosphoglucoronat dehydrogenase G-6-PD  Glucose-6-phosphat dehydrogenase Tf  Transferin

  9. Pewarnaan darah • Sampel darah ? • Darah hewan/manusia ? • Darah hewan ------ hewan apa ? • Darah manusia ---- Golongan apa ? • Dapat ditentukan jenis kelamin/ umur/ ras ? • Jawab: • Uji Kristalin :  Phenolphtalin + Haemoglobin • Peroksidase pink terang • 2 & 3. Uji gel presipitasi:  Prespitin + darah orang • (serum kelinci antihuman) • Klot • 4. Uji serum typing, A, B, O: Antibodi + darah • A, B, O, AB • 5. Test nitrat : -- tidak pasti • perlu uji protein, enzim

  10. Uji serologi

  11. Uji darah untuk kasus kriminal • Darah segar?-- darah kering • Genangan darah • - Tetesan darah/ bercak darah • - Usap darah • - Darah kering

  12. Bercak darah

  13. Usapan darah

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