1 / 23

Exploring Signal Diffusion in A. nidulans: iGEM 2011 Project Proposal

This project proposal from iGEM 2011 at Wageningen UR focuses on investigating the diffusion of signals in the filamentous fungal organism Aspergillus nidulans. The primary aim is to track the diffusion of a fluorescent signal (eGFP) through hyphae and to understand the processes behind signal production and carrier buildup. Through different proposed mechanisms, including the use of the GAL4 system to tune and regulate the signaling processes, the project seeks to lay foundational knowledge on fungal biology and synthetic biology applications. It presents both challenges and opportunities for advancing research in eukaryotic systems.

moshe
Download Presentation

Exploring Signal Diffusion in A. nidulans: iGEM 2011 Project Proposal

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mold Track `n Trace Mold’ly going where no man has gone before iGEM 2011 project proposal – Wageningen UR 2011

  2. A. nidulans Following a signal (eGFP) through a hypha Diffusion of signal carrier through septa

  3. Project - Hyphae

  4. Project - Hyphae Initial stimulation

  5. Project - Hyphae Initial stimulation First signal produced Carrier buildup

  6. Project - Hyphae Initial stimulation First signal produced Carrier buildup Carrier diffuses Process start again in cell N+1

  7. Project - Hyphae Initial stimulation First signal produced Carrier buildup Carrier diffuses Process start again in cell N+1 etc.

  8. Project - Colony

  9. Project - Colony

  10. Project - Colony

  11. Project - Colony

  12. Project - Colony

  13. Project - Colony

  14. Proposed mechanisms xpna eGFP-Svac XlnR* XlnR* XlnR* XlnR* XlnR* XlnR* XlnR* xpna XlnR*

  15. Proposed mechanisms UASgal4 eGFP-Svac GAL4 UASgal4 GAL4

  16. GAL4 regulation 3c 3c 3c 3c 80 80 80 80 80 80 4 4 4 4 4 4 10bp 10bp

  17. Combination 3c 3c 3c XpnA GAL3c 80 80 4 4 XpnA XlnR* eGFP-Svac

  18. S.W.O.T

  19. S.C.O.T New model organism, A. nidulans Visual signal (GFP) System can be subdivided (modular) Proposed RR have been used before Some are even BioBricked In house knowledge about A. nidulans

  20. S.C.O.T Keeping noise from ruining system Tuning the system Lots of constructs need to be made

  21. S.C.O.T Tuning and noise reduction using the GAL system Opportunity to gain fundamental knowledge about A. nidulans hyphae Opportunity to broaden the scope of synthetic biology BioBricks

  22. S.C.O.T Long period from transformation until measuring of results, 1 Month. Unknown synthetic territory Eukaryote chassis

  23. Questions ?

More Related