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Chemistry of Cells. Chapter 2, Section 3. Objectives. Describe the distinguishing characteristics of carbohydrates Describe the important biological functions of polysaccharides Explain what distinguishes lipids from other classes of biological macromolecules

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Chemistry of Cells

Chapter 2, Section 3


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Objectives

  • Describe the distinguishing characteristics of carbohydrates

  • Describe the important biological functions of polysaccharides

  • Explain what distinguishes lipids from other classes of biological macromolecules

  • Describe the unique properties, building blocks and biological roles of fats, phospholipids and steroids

  • Distinguish proteins from the other classes of macromolecules


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Objectives Cont.

  • List the biological functions which proteins perform

  • Explain what determines protein conformation and why it is important

  • Define denaturation and explain how proteins may be denatured

  • Describe the characteristics that distinguish nucleic acids from the other classes of macromolecules

  • Summarize the functions of nucleic acids


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Objectives Cont.

  • Briefly describe the three-dimensional structure of DNA

  • Evaluate the importance of energy to living things

  • Relate energy and chemical reactions

  • Describe the role of enzymes in chemical reactions

  • Identify the effect of enzymes on food molecules


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MacroMolecules

  • Macro = large

  • Molecules = 2 or more atoms covalentlybonded

  • Usually referred to as polymers

    • Like a chain

  • Made from several repeating subunits

    • The repeated subunits are called monomers

    • Like links in a chain

  • 3 of the 4 macromolecules are polymers of monomers


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Making or Breaking Polymers

  • The chemical mechanisms that cells use to make and break polymers are similar for all classes of macromolecules.


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Making Polymers

  • Monomers are connected by covalent bonds via a condensation reaction or dehydration reaction.

    • One monomer provides a hydroxyl group and the other provides a hydrogen and together these form water.

    • This process requires energy and is aided by enzymes.


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Breaking Down Polymers

  • The covalent bonds connecting monomers in a polymer are disassembled by hydrolysis.

    • In hydrolysis as the covalent bond is broken a hydrogen atom and hydroxyl group from a split water molecule attaches where the covalent bond used to be.

    • Hydrolysis reactions dominate the digestive process, guided by specific enzymes.


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Types of Macromolecules

There are four of them.

  • Carbohydrates

  • Lipids

  • Proteins

  • Nucleic acids

    ☺ For each of these you will be expected to identify, describe, and differentiate between all four macromolecules.

    ☺You will also be expected to describe the biological importance of each macromolecule


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Function of Carbohydrates

  • Sugars, the smallest carbohydrates, serve as fuel and carbon sources

  • Polysaccharides, the polymers of sugars, have storage and structural roles


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Structure of Carbohydrates

  • Monosaccharidesgenerally have molecular formulas containing C,H and O in a 1:2:1 ratio.

    • For example, glucose has the formula C6H12O6.

    • Most names for sugars end in -ose.

  • Monosaccharides are also classified by the number of carbons in the backbone.


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  • Monosaccharides, particularly glucose, are a major fuel for cellular work.

  • They are also building blocks for of other monomers, including those of amino acids (protein) and fatty acids (lipids).

  • While often drawn as a linear skeleton, in aqueous solutions monosaccharides form rings.


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2. Polysaccharides, the polymers of sugars, have storage and structural roles

  • Polysaccharides are polymers of hundreds to thousands of monosaccharides joined together (What is a polymer?)

  • One function of polysaccharides is energy storage

    • it is hydrolyzed as needed.

  • Other polysaccharides serve as building materials for the cell or whole organism.


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  • Starch is a storage polysaccharide composed entirely of glucose monomers

    • Great big chain of glucose molecules

    • What would this look like? (Draw it.)


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Biological Uses of Polysaccharides

  • Plants store starch within plastids, including chloroplasts.

  • Plants can store surplus glucose in starch and withdraw it when needed for energy or carbon.

  • Animals that feed on plants, especially parts rich in starch, can also access this starch to support their own metabolism.

  • Hey, this sounds like an objective!


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Lipids - Diverse Hydrophobic Molecules

  • Fats store large amounts of energy

  • Phospholipids are major components

    of cell membranes

  • Steroids include cholesterol and certain hormones


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Introduction

  • Lipids are an exception among macromolecules because they do not have polymers.

    • Though lipid structure is easily recognized

  • Lipids all have little or no affinity for water.

  • Lipids are highly diverse in form and function.


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1. Fats store large amounts of energy

  • Although fats are not strictly polymers, they are large molecules assembled from smaller molecules by dehydration reactions.

  • A fat is constructed from two kinds of smaller molecules, glycerol and fatty acids.


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  • The many nonpolar C-H bonds in the long hydrocarbon skeleton make fats hydrophobic.

  • In a fat, three fatty acids are joined to glycerol by an ester linkage, creating a triacylglycerol.


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Saturated vs Unsaturated the molecule is an

  • Fats with saturated fatty acids are saturated fats.

    • Most animal fats

    • solid at room temperature.

      • Straight chains allow many hydrogen bonds

    • A diet rich in saturated fats may contribute to cardiovascular disease (atherosclerosis) through plaque deposits.

  • Fats with unsaturated fatty acids are unsaturated fats.

    • Plant and fish fats, known as oils

    • Liquid are room temperature.

      • The kinks provided by the double bonds prevent the molecules from packing tightly together.


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2. Phospholipids are major components of cell membranes the molecule is an

  • Phospholipids have two fatty acids attached to glycerol and a phosphate group at the third position.

  • The “head” likes water

  • The “tail” hates water


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  • At the surface of a cell phospholipids are arranged as a bilayer.

    • the hydrophilic heads are on the outside in contact with the aqueous solution and the hydrophobic tails form the core.

    • The phospholipid bilayer forms a barrier between the cell and the external environment.

  • They are the major component of cell membranes.


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3. Steroids include cholesterol and certain hormones bilayer.

  • Steroids are lipids with a carbon skeleton consisting of four fused carbon rings.

    • Different steroids are created by varying functional groups attached to the rings.


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Proteins - Many Structures, Many Functions bilayer.

A polypeptide is a polymer of amino

acids connected to a specific sequence

2. A protein’s function depends on its specific conformation


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Introduction bilayer.

  • Proteins are instrumental in about everything that an organism does.

    • structural support,

    • storage

    • transport of other substances

    • intercellular signaling

    • movement

    • defense against foreign substances

    • Proteins are the main enzymes in a cell and regulate metabolism by selectively accelerating chemical reactions.

  • Humans have tens of thousands of different proteins, each with their own structure and function.


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  • Proteins are the most structurally complex molecules known. bilayer.

    • Each type of protein has a complex three-dimensional shape or conformation.

  • All protein polymers are constructed from the same set of 20 monomers, called amino acids.

  • Polymers of proteins are called polypeptides.

  • A protein consists of one or more polypeptides folded and coiled into a specific conformation


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A polypeptide is a bilayer.polymer of amino acids connected in a specific sequence

  • Amino acids consist of four components attached to a central carbon, the alpha carbon.

  • These components include a hydrogen atom, a carboxyl group, an amino group, and a side chain.

  • Polypeptides are made of amino acids

    • Amino acids CONTAIN NITROGEN (N)


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. bilayer.


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Nucleic Acids sheets.

  • Contain genetic information

    • Provides instructions for making polypeptides

  • Each monomer is a nucleotide

  • Nucleotides are composed of

    • 5 carbon sugar

      • Deoxyribose

      • ribose

    • Phosphate group

    • Nitrogenous base

      • Adenine (A)

      • Thymine (T) in DNA, Uracil (U) in RNA

      • Guanine (G)

      • cytosine


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  • D sheets.eoxyribonucleic acid (DNA)

    • Sugar is deoxyribose

    • Shape is a double helix

  • Ribonucleic acid (RNA)

    • Sugar is ribose

    • Uses a different nitrogenous base

      • Uracil (U) instead of thymine (T)

    • Shape may be a single or double helix