DNA Probing. By: Jennifer Oxford, Jacob Carter, Elemuel Coleman. Table of Contents. What is a DNA Probe? DNA Probe Simplified Process of Searching for DNA/RNA Sequence Important Processes of Hybridization Southern and Northern Blotting Blotting Method Blotting Methods Recap
By: Jennifer Oxford, Jacob Carter, Elemuel Coleman
There are two vital steps of using hybridization: Denaturation and gel electrophoresis.
Denaturation: Heating causes double helix strand to separate.
Gel Electrophoresis-Used in Microbiology. Typically defined by the use of an electric field to move negatively charged nucleic acid molecules through an agarose. This is achieved by moving negatively charged nucleic acid molecules through an agarose gel. Shorter molecules move faster and migrate farther than longer ones.
However, the Northern blot is used specifically for RNA.