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Development of a Modular Peristaltic Microfluidic Pump and Valve System

In our project, we are developing a modular peristaltic microfluidic pump and valve system to enable precise control of fluid flow in biological applications. Our system aims to minimize reagent consumption, increase reaction speed, and study biological phenomena at the single-cell level. The design includes soft lithography for microfluidics and pneumatic valves with low aspect ratios. Our future work includes microfabrication training, extension of control systems, and valve characterization.

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Development of a Modular Peristaltic Microfluidic Pump and Valve System

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  1. Development of a Modular Peristaltic Microfluidic Pump and Valve System BME 272 Group 20: Adam Dyess, Jake Hughey, Michael Moustoukas, Matt Pfister

  2. Microfluidics • Minimal reagent consumption • Increased speed of reactions • Study of biological phenomena at the single cell level

  3. Soft Lithography

  4. Current Pumps at VIIBRE • Harvard Pico Plus syringe pumps • $2,000 / pump • Limiting complexity of microfluidic devices

  5. Pneumatic Valves • Two-layer PDMS device • Flow layer (bottom) • control layer (top) • Thin PDMS membrane deflects into the flow channel when the control channel is pressurized • Low aspect ratios • 1:20 for rectangular control channel • 1:10 for round flow channel Unger et al. 2000

  6. Pneumatic Valve Design • Flow channel dimension – 100um wide, 10um tall (round, AZ p4620, mask) or 5um tall (rectangular, SU8) • Control channel – valve area (300um by 300um), control line 100um wide, 25um-30um tall

  7. Valve in Action

  8. Current Design of Peristaltic Pump • Consists of 4 valves • Round flow channel • Spacing between each valve 100um • Control pressure: 20psi-25psi Issues • Mechanical stability of membrane • Reusability of pumps • Surface adsorption to PDMS • Flow rates and pressures unknown • Gas diffusion through membrane • Minimize peristalsis

  9. Pump in Action

  10. Nitrogen or Liquid Screw or Pneumatic Valves Christmas Tree Nanophysiometer Connector Fluid Flow Peristaltic Pump

  11. Future Work • Learn microfabrication procedures from Yuxin • Get Mike and Adam trained in microfabrication (pending IRB approval) • Extension and documentation of LabVIEW pump control system • Build additional valve control box • Characterize valves and pump

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