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Network in Fluorescence Applications in Biotechnology and Life Sciences. Mark Prescott Department of Biochemistry and Molecular Biology. Genetically encoded chromophores. Structure Develop as useful tools Biological applications. Crystallography approach to 3-Dimensional Structure.
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Network in Fluorescence Applications in Biotechnology and Life Sciences Mark Prescott Department of Biochemistry and Molecular Biology
Genetically encoded chromophores • Structure • Develop as useful tools • Biological applications
Crystallography approach to 3-Dimensional Structure
Crystals of some chromoproteins and fluorescent proteins Collaboration with Jamie Rossjohn, Monash
EqFP611 DsRed Rtms5 • Collaboration with HcRed • Photo-chemist? Alternative chromophore configurations
Rtms5 T-Sapphire Rtms5 Retrieving spectral space chromoproteins as ‘dark’ FRET acceptors for FLIM
‘Chrombodies’ In vivo labeling reagents for FRET/FLIM Grafted CDRs N C In collaboration with Andrew Bradbury – Biosciences Division, Los Alamos
Mitochondrial membrane structure ATP synthase and cristae Probe and model arrangement in vivo using FRAP and FCS
Some challenges • Isolate and characterise further genetically encoded fluorescent labels (far-red emissions). • Understand the post-translational alterations that lead to the optical properties of these proteins (eg: photo-conversion). • Develop genetically encoded chromoproteins for use as ‘dark’ acceptors in FLIM applications. • Develop libraries of ‘chromobodies’ to significantly extend possibilities labeling possibilities in vivo. • Investigate environment / liftetime relationships in vivo for FPs. • Improved sensitivity