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Day 2-Order of the day:

Day 2-Order of the day:. Last observation of your adults Place adults back in original tanks (make sure you check # on tanks) Harvest your embryos or adopt from others who have some Count embryos/record Observe embryos using microscopes-draw in booklet Clean out any debris in petri dish

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Day 2-Order of the day:

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  1. Day 2-Order of the day: • Last observation of your adults • Place adults back in original tanks (make sure you check # on tanks) • Harvest your embryos or adopt from others who have some • Count embryos/record • Observe embryos using microscopes-draw in booklet • Clean out any debris in petri dish • Place petri dish in light/heat box

  2. Microscopes • Dissecting microscope – Easier to use than the other microscopes. Image is not inverted, lower magnification • 2 light sources – one from above and one from below • Different magnifications • Focus knob • May want black felt under petri dish

  3. Egg/embryo care • Observe the breeding tank. Do the fish look different? Do you notice anything on the bottom of the tank besides waste? If so, describe in your manual. • Take breeding tank and return fish to their homes. • Obtain petri dish and fill ½ way with egg water (blue liquid in squirt bottle) • Remove insert and use pipette to gently suck up and transfer eggs to petri dish. • OR pour water through white net (fine mesh). Eggs will gather in net. Invert net, and touch to egg water. They will transfer to the egg water in the petri dish. • Look at your eggs under the microscope

  4. Egg Care • Remove any garbage – Fish waste (poop), broken eggs, unfertilized eggs, etc.. • Count the number of fertilized eggs and record in your manual • Draw eggs and label with Chorion, Embryo, and Yolk (High and Low magnification)

  5. Detailed Recording of genotype within your WHOLE “family” -Drawing must show details of anatomy-label all visible body parts. • -If you have shared embryos, make sure to record which groups received them. You MUST have complete record of ALL fish in your particular “family”. • -If you have adopted your embryos, you are responsible for obtaining ALL results from the WHOLE “family” of zebrafish you adopted from.

  6. Detailed Recording of Embryonic Development-completed daily • -Drawings must show details including labeling of all visible body parts • -Compare your embryos to sheets with embryonic stages-determine age of embryos in hours • -When petri dish contains a range of ages, try and determine the youngest and oldest among your embryos and record. Give possible reasons for this range.

  7. Egg Structure Embryo – Continued cell division eventually leads to a fish. What is the process of cell division called? Yolk – Provides nutrition for the developing embryo Chorion – Provides a protective shell for the embryo Use these terms to label your diagrams

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