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Novel Prochlorococcus ecotypes from the Anoxic M inimum Z one of the Eastern Tropical South Pacific Ocean. Alvaro Munoz Plominsky. Prochlorococcus ecotypes. HLI HLII LLI LLIV. Partensky & Garczarek, 2010. Prochlorococcus ecotypes. HLI HLII LLI LLIV.
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Novel Prochlorococcusecotypes from the Anoxic Minimum Zone of the Eastern Tropical South Pacific Ocean.Alvaro Munoz Plominsky
Prochlorococcus ecotypes HLI HLII LLI LLIV Partensky & Garczarek, 2010.
Prochlorococcus ecotypes HLI HLII LLI LLIV Kettler et al., 2007.
Prochlorococcus ecotypes HLI HLII LLI LLIV Maximum Likelihood 16S+ITS tree Ulloa et al., unpublished
Prochlorococcus ecotypes HLI HLII LLI LLIV Maximum Likelihood 16S+ITS tree Ulloa et al., unpublished
Anoxic Minimum Zones (AMZs) Layer, of a hundred meters thick, where O2 concentrations fall below the 3nM detection limit of the most sensible O2 sensors. Ulloa et al., 2012
Anoxic Minimum Zones (AMZs) Layer, of a hundred meters thick, where O2 concentrations fall below the 3nM detection limit of the most sensible O2 sensors. Ulloa et al., 2012
Anoxic Minimum Zones (AMZs) Layer, of a hundred meters thick, where O2 concentrations fall below the 3nM detection limit of the most sensible O2 sensors. Ulloa et al., 2012
AMZ Prochlorococcus 1st Approach: Metagenomics (Msc. student Marcia Astorga) Characterization of 454 derived Prochlorococcus contigs.
AMZ Prochlorococcus 1st Approach: Metagenomics (Msc. student Marcia Astorga) Characterization of 454 derived Prochlorococcus contigs. 2nd Approach: Single-Cell Genome Sequencing
AMZ Prochlorococcus 2nd Approach: Single-Cell Genome Sequencing
Oxygen (µmol Kg-1) 0 20 40 60 80 100 140 160 180 200 220 AMZ Prochlorococcus 0 Samples were taken from the 2nd Chl a peak at Station 1 (53 m). Samples were enriched by flow cytometry cell sorting. Cryopreserved in glycerol. Sent for whole genome amplification at Bigelow Laboratory for Ocean Sciences. 20 40 Depth (m) 60 80 0 1 2 3 4 5 Chl a (µg L-1)
AMZ Prochlorococcus 1st Approach: Metagenomics (Msc. student Marcia Astorga) Characterization of 454 derived Prochlorococcus contigs. 2nd Approach: Single-Cell Genome Sequencing 3rd Approach: Culture Screening by qPCR Approx. 350 cultures (of 15-20mL), from Big-RAPA Station 1, were maintained in our laboratory!!
AMZ Prochlorococcus 3rd Approach: Culture Screening “General” primers (Synechococcus + Prochlorococcus) 101 bp
AMZ Prochlorococcus 3rd Approach: Culture Screening 92 bp LL V 97 bp LL VI
AMZ Prochlorococcus 3rd Approach: Culture Screening LL V LL VI
AMZ Prochlorococcus 3rd Approach: Culture Screening LL V LL VI
AMZ Prochlorococcus Single-cell genomes will be available... eventually. Genomic data of AMZ Prochlorococcus is also available among metagenomic datasets. Cultures are currently being scaled for future isolation of pure LL V Procholococcus. qPCR methodology will allow screening of samples from the 2013 cruises to the ETNP and ETSP OMZs.
