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Interpenetrating porous networks in HA-based hydrogels

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  1. Interpenetrating porous networks in HA-based hydrogels S. Vanessa Aguilar 2/2/2011

  2. Mimicking Human Tissue • Nature of human tissue • Vasculature and bronchi Brisken et al, J Mammary Gland Biol, 2006 Tosihma et al, Arch HistolCytol, 2004 Tawhai, et al. J. Appl. Phisiol. 2005

  3. Simple hydrogel porous network applied to spinal cord injury model Willenberg et al. JBMR part A, 2006 Prang et al. Biomaterials, 2006

  4. Plan of Work Goal: We aim to mimic tissues that contain two or more networks of pores by creating entwined porous networks within the same hydrogel, allowing for defined cellular control. • Aim 1: Characterize the micro-scale physical parameters of GMHA-based films using 4 different crystallites • Aim 2: Characterize the macro-scale properties of GMHA-based films using 4 different crystallites • Aim 3: • Construct multiple crystalline networks within a single hydrogel construct • Porosity • Diameter of pores • Degree of branching • Mechanical testing • Handling properties • Self Adhesiveness • Swelling ratios • Degradation rate • Micro and macro analysis properties

  5. Aim 1: Experimental Set Up • Keep the hydrogel material constant and change processing CHAPS urea Potassium dihydrogen phosphate β-Cyclodextrin Courtesy of Scott Zawko

  6. Aim 1: Different crystallites GMHA-CHAPS Alginate - kdp GMHA – kdp Alginate – βcd GMHA - urea GMHA - urea Courtesy of Scott Zawko

  7. Aim 1: Micro Structure Characterization Imaris Filament Tracer Fractal Analysis Jha, A.K, et al, Macromolecules, 2009 [1] Kim and Chu, JBMR, 2000 Wang and Chau,Soft Matter, 2009

  8. Aim 2: Macroscopic characterization http://news.thomasnet.com/news/sensors-monitors-transducers/sensors-detectors/force-load-strain-sensors/compression-tension-sensors/20

  9. Aim 3: Dual crystal templating Goal: Create hydrogel construct with two independent but interwoven porous networks. Shown below: Variety of cell seeding for each network

  10. Acknowledgments PI: Dr. Christine Schmidt Post-Doc: Dr. ZinKhaing Dr. John Hardy Graduate Students: Sarah Mayes Richelle Thomas John Fonner