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Lab 28 Goals and Objectives: Exercise 33: Effectiveness of Alcohol as an Antiseptic

Lab 28 Goals and Objectives: Exercise 33: Effectiveness of Alcohol as an Antiseptic Calculate percent reduction for control, dip and swab Exercise 36: Effectiveness of Hand Scrubbing Calculate CFU/ml for each scrubbing round: don’t forget to multiply by dilution factors:

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Lab 28 Goals and Objectives: Exercise 33: Effectiveness of Alcohol as an Antiseptic

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  1. Lab 28 Goals and Objectives: Exercise 33: Effectiveness of Alcohol as an Antiseptic Calculate percent reduction for control, dip and swab Exercise 36: Effectiveness of Hand Scrubbing Calculate CFU/ml for each scrubbing round: don’t forget to multiply by dilution factors: 0.1ml → x 10 (0.1ml is 1/10 of 1ml) 0.2ml → x 5 (0.2ml is 1/5 of 1ml) 0.4 ml → x 2.5 (0.4ml is 2/5 of 1ml) Exercise 34: Antimicrobial Sensitivity Testing Measure zones of inhibition (total diameter in mm) Record millimeter diameter on class data chart Determine if organism is resistant, sensitive, or intermediate Exercise 35: Evaluation of Antiseptics Note different effects Gram + vs Gram – for some agents

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