Human Genomic DNA Isolation. Zelha Nil Nov 2009. DNA Structure. Composed of nucleotides : A, T, G, C Synthesized in 5’ to 3’ direction through formation of phosphodiester bonds betw deoxyribose & phosphate : Sugar - Phosphate backbone
One from each parent: non-identical
Euchromatin: Lightly packed form of chromatin that is rich in gene concentration
Hypotonicbufferforlysis of RBC and WBC enhancedbyinversions.
Added 2-3 volumes of thesolutiontocollect DNA (orprecipitatenucleicacids) fromaquousphases since nucleicacidstendtoinsolubilize in ethanol.
Dissolve DNA andstoragebuffer