Mice mated a/a dam X Avy/a sire while the dam is on control or 3SZM diet produce Avy/a and a/a offspring. Avy/a offspring have different distributions of epigenetic phenotypes in the population due to maternal diet. .
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Mice mated a/a dam X Avy/a sire while the dam is on control or 3SZM diet produce Avy/a and a/a offspring. Avy/a offspring have different distributions of epigenetic phenotypes in the population due to maternal diet.
Methyl metabolismshowing some major metabolic intermediates, cofactors and dietary sources of methyl groups and an intersection with antioxidant metabolism through homocysteine, cysteine and glutathione.
Survival declines over a considerable range in adult humans (40 to 100 y of age) and in some inbred rodent strains (18 to 33 mo of age, scales are approximate). (Lower panel) Epigenetic traits are probably distributed unevenly and over a considerable range in populations.
Agouti gene expression in the Avy allele. TOP: When the LTR is active, it drives expression of the agouti gene in Avy mice. Transcription is constant, rather than cyclical (indicated by the bold arrow) and is ectopic (i.e., in all tissues studied). BOTTOM: When the LTR is suppressed in Avy mice, expression of the agouti gene is regulated by its normal promoters, is cyclic and occurs only in the hair follicles (indicated by the narrow arrow). LTR, long term repeat.
Map of the Avy allele showing the LTR, agouti exons, primer positions and HaeII (H) and HinP1I (P) sites. IAP, intracisternal A particle; LTR, long terminal repeat.
Cooney et al. (2002) Maternal Methyl Supplements in Mice Affect Epigenetic Variation and DNA Methylation of Offspring. Journal of Nutrition 132, 2393S-2400S
GT CG AATTGTTTATGTTG CG AGTTG
Solvent percentage does not affect measurement of percent methylation using completely volatilebuffer.
Methylation of locus 224 in several tissues (A-E) in two types of epigenetically different mice
Lyko et al 2000
Cooney et al unpubl
BOTTOM: black (a/a) mouse
BOTTOM: Y0 (pseudoagouti Avy/a) mouse.
RIGHT: Y4 (slightly mottled Avy/a) mouse.