Chapter 1 Introduction. Basic rules in practical lesson:. No being late, no absence Bring textbook and so on Take care of your microscope, computer and slides Don ’ t use computer randomly Some functions are forbidden
Don’t use computer randomly
Some functions are forbidden
You can only use it to watch samples , receive and look through my coursewares (PPT),
Ⅰ. A. Master the use of the microscope. B. Familiar with the general structure of the light microscope:
composed of mechanical and optical parts.
Ⅱ. Observation: tissue sections (spinal ganglion)
stage adjustment knobs.
light adjustment konb
Fine focus konb
Coarse focus konb
Skip to Magnification Section
Turn to let more light in or to
Move the stage and retainer left and right,back and forth
So the object is 400 times “larger”
Objective Lens have
written on them.
Ocular lenses usually magnifies by 10x
on the stage with its coverslip turned upward. Move the object in the center by turning the m stage adjustment knobs.
Low magnification(4×, 10×):1. Raise the stage by rotating the coarse knob slowly to bring the slide close to the objective(1mm) while keep watching them from the side of the stage with naked eyes.2. Look into the eyepieces and slowly lower the stage with coarse knob until the image appears and is almost in focus.3. Rotate the fine knob back-and-forth to get exact focusing ( sharp) image.
Before use high power objective Make sure:
Use only the fine adjustment knob under high magnifcation.
naked eye -- lower power-- higher power1. The naked eye examination will get some information of the general shape, size and staining of the section, and of the location of the coverslip.2. Lower power observation will identify the mail structure and overall histologic features of a given section, therefore will find out what it is.3. Higher power observation should be used only for detail work, for example, the microscopic structure of cells.
A number of artifacts that appear in stained may result from improper procedures for making samples (fixation, dehydration, embedding, sectioning and so on )
Artifacts in tissue sample contain tearing artifacts, holes, bubbled appearance, wrinkled appearance,
Space , dye deposits and so on, you should try to distinguish them from normal compnents.