Konan Peck. Tsann-Long Su. Joanne Chen. Cheng-Wen Wu. Yuh-Shan Jou. Steve R. Roffler. Joint appointment, NHRI. You-Di Liao. Sheau-Yang Shieh. Pan-Chyr Yang. Joint appointment, NTUH. Cancer Research Group. Cancer Research Group. A pipeline integration of
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Steve R. Roffler
Joint appointment, NHRI
Joint appointment, NTUH
Cancer Research Group
A pipeline integration of
technology development, basic, applied, and clinical studies
Genomic & proteomic research tool developments
Molecular pathogenesis studies
Carcinogenesis & metastasis
Identification of biomarkers
for diagnosis, prognosis and therapy
Therapeutic agents and clinical assays development
High throughput transcript-specific expression profiling system
Aptamer arrays for protein-specific expression profiling
Model cell lines & multi-well assay for cancer cell invasion studies
(Pan-Chyr Yang & Cheng-Wen Wu)
Bioinformatics tools and database
for hepatocarcinoma oncogenomics
Surface expressed proteins for therapy and imaging
Directed molecular evolution to
create new therapeutic proteins
Cancer genomics & tumor biology of GI-track cancer(Joanne Chen)
1. Overexpression of RTKN, a Rho GTPase effector, contributes to GC pathogenesis,
and its overexpression is associated with GC progression.
2. Abnormal expression of OPN and its receptor CD44 contributes to cancer progression.
Identify and study human hepatocellular carcinoma genes
by genomic approaches (Yuh-Shan Jou)
- HCC genes somatic mutation screening
- Homozygous deletion of chromosome 13q12.11
- Integrated HCC database: OncoDB.HCC for bioinformatics analysis
Genome-wide minimal deleted regions, two major tumorigenic pathways,
and a homozygous deletion of 13q12.11 in HCC have been identified.
J. Biol. Chem. 280: 7748-7757, 2005
Mol. Biol. Cell 16:1684-1695, 2005
Molecular pathogenesis studies (carcinogenesis)
p53 and cell cycle checkpoints(Sheau-Yann Shieh)
- The role of CHK1, CHK2-interacting proteins in checkpoint control
- The role of CHK1 and CHK2 in DNA double strand break repair
Pathogenesis of human cytomegalovirus(Cheng-Wen Wu)
- The functions of the HCMV IE2 protein
P = 0.019
Probability of disease free
Time in months
Molecular pathogenesis studies (metastasis)
PPG to decipher the molecular mechanism of cancer metastasis
- Genome-wide identification of metastasis related genes(Pan-Chyr Yang & Konan Peck)
- Identification of metastasis-related proteases and protease inhibitors(Cheng-Wen Wu)
- Characterize novel and known invasion/metastasis suppressors and enhancers:
CRMP-1, HLJ1, LCRMP-1, Slug(Pan-Chyr Yang);
TAL6 (Steve Roffler)
KLK8 (Konan Peck)
Empirical approaches for validation and characterization
Cell biology studies
Mouse model studies
In silico database search
Empirical genomic approaches
Candidate marker genes
Validation with cancer cell lines
Validation with mouse models
Validation with clinical specimens
Metastasis and prognosis biomarkers:
CRMP-1, LCRMP-1, HLJ1, Slug, KLK 8
TAL6, CD13, CD44, RTKN, OPN
Cancer therapy efficacy assessment markers:
KRT19, UCHL1, FN1, TRIM28
Drug target for metastasis inhibition:
Therapeutic agent developments
Discovery and Development of Potential Anticancer Agent
(Tsann Long Su)
Gene alkylating agents
(in preclinical studies)
Topoisomerase II Inhibitors
• Topoisomerase I Inhibitors
Glucuronide prodrugs for cancer therapy
Anti-tumor Ribonucleases from Bullfrogs
Microarray for prognosis of NSCLC patients
Microarray for subtyping of gastric cancers
Rapid assessment of therapy efficacy and relapse detection method
(Konan Peck & Pan-Chyr Yang)
Using marker genes to detect the amount of circulating tumor cells before and after the treatment allows rapid efficacy assessment and relapse detection.
Publications in academic journals
Technology transfer to biotech industry
Publications in basic research journals
American Journal of Respiratory Critical Care Medicine
American Journal of Pathology
Journal of Biological Chemistry
Journal of the National Cancer Institute
Molecular Biology of the Cell
The EMBO Journal
Publications in applied or clinical research journals
Clinical Cancer Research
Journal of Clinical Oncology
New England Journal of Medicine
Nucleic Acids Research
Multi-national intellectual property rights
Pending patent applications
P.C. Yang, et al. (2003) European Patent 1271153 A2
P.C. Yang, et al. (2003) US Patent # 20030077624 A1.
P.C. Yang, et al. (2003) European Patent 1285970 A2
P.C. Yang, et al. (2003) US Patent # 20030054387 A1.
S.R. Roffler, et al. (2004) US Patent # 20040014156 A1.
T.L. Su, et al. (2004) US Patent # 20040198765 A1.
P.C. Yang, et al. (2005) US Patent # 20050182016 A1.
Y.D. Liao, et al. (2005) US Patent # 20050214899 A1.
K. Peck, et al. (2001) US Patent# 6,218,114
K. Peck, et al. (2001) ROC patent 438889
K. Peck, et al. (2002) European Patent EP 0822261
K. Peck, et al. (2003) ROC patent 541340
S.R. Roffler, et al. (2003) US Patent # 6,596,849
S.R. Roffler, et al. (2003) US Patent # 6,617,118
S.R. Roffler, et al. (2003) European Patent EP 0990661
S.R. Roffler, et al. (2004) US Patent # 6,699,972
S.R. Roffler, et al. (2004) ROC Patent 205511
T.L. Su, et al. (2004) US Patent # 6,821,983
K. Peck, et al. (2005) US Patent # 6,867,050
K. Peck, et al. (2005) ROC patent I232867
Technology transfers to biotech industry
1. S.R. Roffler, et al. “Monoclonal antibody against polyethylene glycol” to Bayer and Hoffmann-LaRoche
2. K. Peck, et al. “Method for profiling gene expression pattern and isolation of differentially expressed genes”
to Taiwan Genome Sciences, Inc. and 4 other companies.
3. K. Peck, et al. “Method for constructing high-density non-redundant gene arrays”
to Taiwan Genome Sciences, Inc. and 2 other companies.
4. K. Peck, et al. “Methods for rapid antimicrobial susceptibility testing” to Cold Spring Biotech, Inc.
5. P.C. Yang, et al. “Metastasis-associated genes” to AdvPharma, Inc.
6. P.C. Yang, et al. “Collapsin response mediator protein-1 as tumor metastasis marker” to AdvPharma, Inc.