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Agrobacterium-Mediated Genetic Modification in Carrot Cells

Explore the process of cellulose synthesis and T-DNA transmissibility in carrot cells influenced by Agrobacterium. Study the function of oncogenes, vir genes, opine synthesis, and catabolism genes involved in plant wound healing. Uncover the significance of phenolic compounds in T-DNA transfer and understand the transcriptional regulation of vir genes including VirB in the interaction between Agrobacterium and plant cells.

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Agrobacterium-Mediated Genetic Modification in Carrot Cells

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  1. C58 attached to the surface of carrot cells

  2. Cellulose synthesis by A. tumefaciens

  3. T-DNA Transmissability genes Fig. 24-40 Oncogenes Opine synthesis vir genes (encode virulence factors) Opine catabolism genes

  4. Plant wound Phenolic compounds T-DNA T-DNA Single- stranded nick Agrobacterium cell Fig. 24-41 Transcription of other vir genes VirB Plant cell Agrobacterium cell

  5. Figure 16.31

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