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A B. 1 00 80 60 40 20 0. 1 00 80 60 40 20 0. isotype parental DR4 overexpression. isotype parental DR5 overexpression. events. events. 0 10 2 10 3 10 4 10 5 DR4-Alx647. 0 10 2 10 3 10 4 10 5 DR5-Alx647.

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slide1

A

B

100

80

60

40

20

0

100

80

60

40

20

0

isotype

parental

DR4 overexpression

isotype

parental

DR5 overexpression

events

events

0 102 103 104 105

DR4-Alx647

0 102 103 104 105

DR5-Alx647

50

25

0

NS

NS

NS

specificapoptosis (%)

TRAIL  + + + ­

parental DR4 overexpression DR5 overexpression

SupplementaryFigure 1

slide2

A

*

*

*

*

*

*

50

25

0

specificapoptosis (%)

parental p53 p53p53p53p53p53

wt V143A R175H R248W R249S R273H

p53-FKBP

p53

GAPDH

SupplementaryFigure 2

slide3

B

*

*

*

*

*

50

25

0

*

specificapoptosis (%)

parental p53 p53p53p53p53p53

wt V143A R175H R248W R249S R273H

SupplementaryFigure 2

slide4

CEM

A

*

100

75

50

25

0

specificapoptosis (%)

mock shp53

p53

GAPDH

SupplementaryFigure 3

slide5

B

25

0

SKW6

REH

specificapoptosis (%)

mock shp53

25

0

p53

NS

NS

50

25

0

NS

NS

GAPDH

25

0

SW48

Nalm6

specificapoptosis (%)

specificapoptosis (%)

specificapoptosis (%)

mock shp53

p53

p53+/+ p53-/-

mock shp53

GAPDH

p53

p53

SupplementaryFigure 3

GAPDH

GAPDH

slide6

C

*

*

*

100

75

50

25

0

A549

JURKAT

50

25

0

specificapoptosis (%)

specificapoptosis (%)

50

25

0

H1299

mock shp53

mock shp53

p53

p53

GAPDH

GAPDH

specificapoptosis (%)

p53-/- p53 wt

p53-FKBP

GAPDH

SupplementaryFigure 3

slide7

0

-25

-50

-75

-100

Relative reductionof p53 expression in

p53 siRNAtransfectedcells (%)

ALL-10S ALL-54 ALL-177

SupplementaryFigure 4