The Growth of Bacterial Cultures. Ways of monitoring and measuring growth, directly and indirectly. Reproduction in Prokaryotes. Bacteria normally reproduce through Binary fission
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-Time required for cell to divide (and its population to double)
Then, 5 x 29 = 2560 cells
Refer to Appendix D of your textbook
= log 1,720,320 – log 100 = 6.2356 - 2 =14 generations
= 60 minutes x 5 hours = 21minutes/generation
*These calculations are useful to compare growth conditions
Figure 6.15, top portion
Optimal number to count is 30-300 colonies
Figure 6.17a, b
600nm to measure Pichia growth
-measure product as a function of time
Example: Bacterial culture produces acid as a metabolic product, therefore you can monitor growth by monitoring change in pH over time.