Feasibility Study of Pandanus odoratissimus L. as Flea Repellant

1. Y O U T H C A N 2014 Feasibility Study of PandanusodoratissimusL. as Flea Repellant PRESENTER Christian A. Salino Third Year Student Quirino High School, Quezon City Philippines and at AMERICAN MUSEUM OF NATURAL HISTORY, New York, USA April 21, 2014

2. Co-advisers: Ms. Rosalina L. Molina Mrs. Lucila V. Dizon Ms. Madeline S. Cuevas Adviser:Mr. Rolando R. Barcelon

3. Feasibility Study of Pandan Leaves Extract (PandanusodoratissimusL.) as Flea Repellant CHAPTER 1

4. Background of the Study • Pest is any organism that causes nuisance and epidemic disease associated with high mortality. Malaria Dengue

5. Background of the Study • Some people that are concerned in repelling fleas invented natural products and found ecological-friendly ways that will drive the fleas off.

6. Statement of the Problem • Pandan Leaves (Pandanus odoratissimusL.) extract contain property that can drive-off fleas? 2. Is there any significant effect in the number of fleas on the dog’s body?

7. Objective of the Study • To study the response of the dog’s fleas to the application of the extract from the pandanleaves

8. Statement of the Hypothesis • Pandanleaves extract is not effective as flea-repellent agent. • There is no significant effect on the number of fleas in the dogs’ bodies from the extract of pandanleaves after the different frequencies of the treatment.

9. Significance of the Study • To our countrymen • Pets • Environment

10. Scope and Limitations of the Study • Use of extract from the leaves of pandan in repelling fleas. • Quantitative comparison on the effectiveness of the different concentrations of pandanleaves as anti-flea agent.

11. Chapter 2 REVIEW OF RELATED LITERATURE AND STUDIES

12. According to the Study of WikiHome

13. WikiHome • Use botanical dust mixed with Borate acid.  • Use a lemon solution in affected areas.  • Clean your pet regularly. • Add deterrents to the pet's meal. Source:http://www.wikihow.com/Kill-Fleas-in-a-Home

14. Safe, Effective Flea ControlCombat Fleas Outdoors Too by Richard H. Pitcairn, DVM, PhD

15. Safe, Effective Flea ControlCombat Fleas Outdoors Too • Use of herbal flea powder • Use of herbal flea collar

16. 5 Herbs That Naturally Repel Fleas • Citronella • Catnip • Marigold • Lavender • Peppermint

17. Pandan Leaves (PandanusodoratissimusL.)

18. Chapter 3 Methodology

19. Brief Description of the Equipment • Knife - used for slicing the leaves into smaller pieces • Chopping Board - a board where leaves are sliced into smaller pieces • Weighing Scale - weighs the amount of leaves needed for decoction • Measuring Glass - measures the volume of water needed for decoction • Kettle - container used for decoction of the mixture • Basin - used as container for the sliced pandan leaves • Hand Sprayer - used as application tool • Stove - supplies heat for decoction

20. General Procedure Extraction 1. Researcher gather pandan(PandanusodoratissimusL.) leaves to be sliced into smaller pieces using knife and chopping board.

21. General Procedures 2. Weigh the amount of smaller pieces using the weighing scale into the basin and put it in the kettle with an amount of water using the measuring glass.

22. General Procedures 3. Heat the kettle with the use of the stove for thirty minutes (30 minutes) for decoction.

23. General Procedures 4. Let the kettle be cold and squeeze the pandan leaves (PandanusodoratissimusL.) to get its extract. Set aside the squeezed pandanfor future use.

24. General Procedures Application • Researcher look for three (3) dogs which suffer from fleas. • The first dog will be treated with 1 kilogram (1 kg) of pandan leaves (PandanusodoratissimusL.) decocted in 1000 mL of water, as Treatment A, using hand sprayer. • The second will be treated with 1 kilogram (1 kg) of pandan leaves (PandanusodoratissimusL.) decocted in 1500 mL of water using hand sprayer, as Treatment B. • The third dog will be treated with 1 kilogram (1 kg) of pandan leaves (Pandanusodoratissimus) decocted in 2000 mL of water, as Treatment C, using hanL.dsprayer.

25. Partial picture of the respondent using the first treatment in trial 1 with 1 kilogram of pandan leaves that decocted in 1000 mL of water. Treatment 1 The first dog that was treated with the treatment A with the helped of the owner.

26. Chapter 4 Results and Interpretation of the Data

27. Results and Interpretation of the Data Table 1.1 Treatment A of Pandan extract to Dog No. 1

28. Results and Interpretation of the Data Table 1.2 Treatment B of Pandan extract to Dog No. 2

29. Results and Interpretation of the Data Table 1.3 Treatment C of Pandan extract to Dog No. 3

30. Results and Interpretation of the Data • It shows that in every trial, there were decreasing numbers of repelled fleas. The dog was treated once a day in 5 consecutive days as represented by the number of trials. There is an evident decrease on the number of fleas on the dog after every treatment

31. Results and Interpretation of the Data

32. Results and Interpretation of the Data • Treatment A has the highest flea-repelling effect followed by Treatment B then Treatment C.

33. Chapter 5 Conclusion and Recommendations

34. Conclusion The researchers documented that the more concentrated pandan leaf extract is further effective than the diluted one, thus the effectiveness rises when the degree of concentration increases.

35. Recommendations • That the pandan leaf extract be tested to a wide variety of host animals that are also susceptible to dog fleas (Ctenocephalidescanis) like domestic cats and other mammals. • That the propagation of Pandan (PandanusodoratissimusL.) be sustained for future pharmacological intervention on both animals and human.

36. Recommendations • That in the depth study on the flea-repelling properties of Pandan (PandanusodoratissimusL.) be undertaken hence the determination of the active repelling substance. • That further research to identify the repelling ingredient of the Pandan (PandanusodoratissimusL.) against other pests and insects.

37. The Presentation Adviser Mr. Rolando R. Barcelon, (Master Teacher I of the Science Department) QHS SEED Coordinator for Student Affairs

38. The Presentation Co-adviser Mrs. Lucila V. Dizon, (Teacher III of the English Department) Our very energetic, approachable, and supportive, QHS SEED Coordinator for Communication

39. The Presentation Co-adviser/Coordinator Ms. Rosalina L. Molina, (Master Teacher II of the Mathematics Department) Our very friendly, dynamic, approachable, and energetic, QHS SEED Coordinator

40. The Presentation Co-adviser/Coordinator Ms. Madeline S. Cuevas, (Teacher III of the Science Department) Very benevolent, friendly and energetic science teacher

41. Mrs. Carolyn C. Simon • (Head Teacher III) • & • Ms. Cecilia H. Paguia, • (Head Teacher III) • our Science and Math Department Heads • respectively - • for their sustaining guidance and support

42. Our loving, kind and approachable school principal • Dr. LEVITA U. RAMOS • School Principal IV, Quirino High School, QC Phil.

43. Our very supportive Ma’am Corinne Damas-Sandiford Project Manager, Schlumberger  SEED Paris Our supportive, kind Ma’am Gloria Bato, SEED Coordinator, Philippines

44. Schlumberger Excellence in Educational • Development (SEED) and staffs headed by • Simone Amber for the magnificent opportunity given to us to share and the benevolent contributions in educating the youth worldwide; • special thanks to Ma’am Monia, Ma’am Susan Randel & Sir Nathan Bellomy for their help and encouragement.

45. friends, classmates, schoolmates; Si-SEED-lan Club

46. A million thanks • to our loving GOD, • for the continued wisdom, strength or blessings showered to • to us to finally present this • presentation internationally.