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Entry 40 11/5 • CSI – What does CSI stand for? • List everything you know about CSI. • Hypothesize about the relationship between CSI and DNA.
G. DNA Fingerprint • DNA fingerprint- A unique band pattern made of DNA fragments. • Unique to every individual, unless you have an identical twin
Uses for DNA Fingerprinting: • Violent Crimes – determines source of DNA left at a crime scene. • Paternity - used to determine the father of a child
Gel Electrophoresis- tool used to create a DNA fingerprint, it separates pieces of DNA based on size. • Size = the number of base pairs in each piece.
Steps in DNA Fingerprinting • Step 1: Restriction enzyme cleaves the DNA sample at a specific sequence. • Restriction enzyme: the enzymes that “cuts” the DNA between the nitrogen bases • Cleave: to Cut (between nitrogen bases)
Step 2: DNA fragments are loaded into wells on a gel. • Step 3: Bands are created as electricity forces DNA fragments through the gel. Small pieces move further than larger pieces. • Step 4: Compare to other DNA samples. http://learn.genetics.utah.edu/content/labs/gel/
Example Semen Sample was retrieved from a rap kit completed at the hospital. The police have 3 possible suspects. A DNA fingerprint has been done to narrow done the suspect pool. Who is the polices MAIN Suspect?
Is the male in the fingerprint the father of the girl?
Ronald Cotton http://www.youtube.com/watch?v=u-SBTRLoPuo
Base Crime Suspect Suspect Suspect Suspect Pairs Data 1 2 3 4 35 34 33 32 1
Ticket out the Door 1. Why are restriction enzymes important when making a DNA fingerprint? 2. What do the bands represent on a DNA fingerprint? 3. Using the fingerprint to the right who is a possible suspect for murder?
Do Now… • Take out 5.1 & 5.2 Bio Apps and Something to grade with
After Test… • Turn in test and pick up Practice Fingerprint when finished with Fingerprint begin reading the Case Study • Once everyone is finished with test we will begin work on a Case Study. • Answer questions on page 2 in your journal (give this entry a title) • Answer COMMON QUESTIONS on page 3 in your journal. • Answer only the questions for your INTEREST GROUP
Entry 41 11/7 Vector Structure used to carry foregin DNA; bacterial plasmids and virus are commonly used Use in a sentence or Draw a picture Transgenic Organism Organism containing foreign DNA Use in a sentence or Draw a picture
Agenda • Notes • Practice Activity • Bio Apps • Quiz the last part of Class tomorrow
H. Genetic Engineering • Genetic Engineering: Modifying DNA or creating recombinant DNA. • Transgenic Organism: an organism which contains foreign DNA from another species. • Recombinant DNA: form of artificial DNA that is created by combining two different sources of DNA. A gene isolated from a species of jellyfish which causes fluorescence was introduced into marmoset embryos that allows them to build green fluorescent protein (GFP) in their tissues. Which glows green when exposed to blue light.
Transgenic Plant • Tobacco plant with firefly DNA Or in some case • Tobacco plant with jellyfish DNA
Process in creating Transgenic organism: • Step 1: Restriction enzyme is used to cleave the desired gene from a DNA sequence (ex. Insulin).
Step 2: The same restriction enzyme is used to cleave the vector. • Vector: The structure used to carry the foreign DNA, bacterial plasmids are commonly used. • Plasmid: Circular DNA found in bacteria
Step 3: Foreign DNA and Vector spliced together • Splice: Combine
Step 4: The recombinant DNA is inserted into the host (bacteria cell). Then the host cell will copy and produce the protein. • Insulin can be produced in large quantities by using genetic engineering and bacteria.
Genetic Engineering • Recombinant DNA http://www.bioteach.ubc.ca/TeachingResources/Applications/GMOpkgJKloseGLampard2.swf
I. Bioethical Concerns for Genetic Engineering • Should we produce artificial proteins? • Allergic reactions (adding a peanut gene to a corn plant) • Stem cell research • Environmental problems from creating transgenic organisms: • Super weeds • Antibiotic resistant bacteria • Destruction of beneficial insects like bees by pesticides
Ticket out the Door • Foreign DNA is spliced into what type of organism? • What is a common use of Recombinant DNA? • Tobacco plant displays a bioluminescence (glows) due to a protein found in fireflies which controls the fireflies luminescence? Which is the transgenic organism Tobacco plant or firefly?
Pick up glue and scissors on LAB STATION 1 • Turn in your assignment from yesterday on the demo desk up front. • Take out your journal.
Entry 43 11/9 • Foreign DNA is spliced into what type of organism? • What is a common use of Recombinant DNA? • Tobacco plant displays a bioluminescence (glows) due to a protein found in fireflies which controls the fireflies luminescence. Which is the transgenic organism tobacco plant or firefly?
PART 3 – Transgenic Organisms(complete on the back) • Instructions: Using what you have learned in this activity and class you willchoose one of the scenarios on the cards and defend your opinion. This will be in summary form (NO BULLETS). You will first identify the scenario you are defending, explain why you agree or disagree with the process (provide two reasons), then support your reasoning with scientific evidence (there should be two pieces of scientific evidence).
Harvest of Fear http://www.youtube.com/watch?v=6NsI0ba9dNg
Ticket out the Door • What is a use of Gene Therapy? • What was the purpose of the Human Genome Project?
Ticket out the Door • Foreign DNA is spliced into what type of organism? • What is a common use of Recombinant DNA? • Tobacco plant displays a bioluminescence (glows) due to a protein found in fireflies which controls the fireflies luminescence? Which is the transgenic organism Tobacco plant or firefly? • What was the purpose of the Human Genome Project?
Entry Cleave To cut My picture My sentence Syn or Ant Splice To attach My picture My sentence Syn or Ant
Flashcard Warm-up Restriction enzymes Enzymes that will cut DNA at specific places. This can be used to create DNA fingerprints or to insert genes through gene therapy. My picture: My definition: Gel Electrophoresis Tool used to create a DNA fingerprint My picture: My definition:
Flashcard Warm-up Recombinant DNA Genetic Engineering Technology that combines DNA from two different organisms. One practical application of this process is making human insulin for people with Diabetes.
Flashcard Warm-up Transgenic Organism Organism containing foreign DNA My Picture My Sentence Vector Structure used to carry foregin DNA; bacterial plasmids and virus are commonly used My Picture My Sentence