1 / 3

Analysis of Mouse and Human Macrophages Using Semiquantitative RT-PCR and Flow Cytometry

This study utilizes semiquantitative RT-PCR and flow cytometry to analyze the ratio of mouse and human macrophages, highlighting the induction process and distinct markers. The impact of an Anti-CD47 antibody on PEL cell proliferation is also evaluated.

lovey
Download Presentation

Analysis of Mouse and Human Macrophages Using Semiquantitative RT-PCR and Flow Cytometry

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Table S1. Primers used for semiquantitative RT-PCR

  2. 4 4 10 10 3 3 10 10 93.4 96.6 2 2 10 10 1 1 10 10 0 0 10 10 0 1 2 3 4 0 1 2 3 4 10 10 10 10 10 10 10 10 10 10 Fig. S1. Representative flow cytometric profiles with the ratio of mouse and human macrophages after the induction of macrophages as described in Material and methods. Human macrophages Mouse peritoneal macrophages CD56 CD49b CD11b CD14

  3. Fig. S2. Anti-CD47 Ab did not inhibit the proliferation of PEL cells. PEL cell lines (BCBL-1, BC-2 and BCP-1) were incubated with 0, 0.3, 1, 3, 10 µg/ml anti-CD47 Ab for 72 h. A cell proliferation assay was carried out using MTT as described in Material and methods. Cell viability(%) Anti-CD47 Ab (μg/ml)

More Related