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Spatio-temporal distribution and genetic characterization of some marine macroalgae of the Republic of Mauritius . MPhil/PhD Research By Mrs Persand Jayshree (BSc Hons Biology with Environmental Sciences) Supervisors:Dr Bhagooli & Dr Taleb-Hossenkhan.
By Mrs Persand Jayshree
(BSc Hons Biology with Environmental Sciences)
Supervisors:Dr Bhagooli & Dr Taleb-Hossenkhan
Studies on macroalgae distribution based on morphological characteristics: Dickie, 1875, data collected during 1860’sBoergesen 1940-1957, data collected during late 1920’s & 1930’s Jagtap, 1993, data collected in 1987
Correct identification at molecular level absolute pre-requisite. Wrong identification misleading for research work
Only short-term studies on species distribution and abundance reported
Identification of macroalgae heavily reliant on
Morphology of a single species vary in response to environmental conditions, for example low salinity and salinity shocks creating morphotypes.
Molecular genetic tools to identify morphotypes.
low salinity and salinity shocks can induce branching in Ulva intestinalis
(U. intestinalis being unbranched) creating morphotypes similar to Ulva compressa.
U. intestinalis and U. compressa are two distinct, genetically divergent and reproductively isolated species
Mschigeni (1985) highlighted
level of misidentification of specimens in some areas in the Indian Ocean may have an adverse effect on other studies and the commercial application of these specimens
This study proposes a re-evaluation of macroalgae identification, abundance and distribution in the Mauritian lagoons and help identify possible effects on macroalage in our lagoons of the recent development that have occurred along the coastal shoreline in the recent years in Mauritius.
Sites of study
very high commercial value worldwide and are being harvested in millions of tonnes annualy
Well-established genetic tools and markers to identify macroalgal species
Genetic analysis of macroalgae
Chl a fluorescence determined each season using Pulse Amplitude Modulated (PAM) fluorometer
(recording the time taken for algal re-colonization in cleared area)
Specific growth rates of macroalgal species monitored
length / mass 30-50 individuals tagged (diameter and length)
2/yr water samples to be collected for nitrate & phosphates analyses ex situ (cadmium reduction method & ascorbic acid method)
water physico-chemical parameters including temperature, pH, salinity, turbidity & dissolved oxygen monitored in situ
4 equidistant transects perpendicular to shore evenly spaced macroalgae distribution and seasonal change monitored
Wet weight: 3 random samples in 25cm 25cm quadrat/transect
Dry weight: same samples dried at 800c
suitability of certain macroalgae as bioindicators ???
Figure 1: Organisation of one rDNA array. Single repeat units (arrows) are tandemly organised. Each of them consists of the rRNA genes: 18S, 5.8S and 28S. Spacers separate these genes, namely the external transcribed spacer (ETS), the internal transcribed spacers (ITS 1 and ITS 2) and the intergenic spacer (IGS). [http://webdoc.sub.gwdg.de/ebook/y/1999/whichmarker/index.htm]
Multiple Sequence Alignments (MSAs) deriving from different Ulva spp. and of the rubisco gene cluster for Gracilaria spp. were then produced using the MultAlin Software
Primers, specific for the amplification of the polymorphic regions, were then designed using Primer3WWW software
for your consideration