Linkage, Recombination and Eukaryotic Mapping. Part 2. Outline. Gene mapping in Humans Pedigrees and linkage Physical Chromosome mapping. Nail-Patella Syndrome. Autosomal dominant: abnormal fingernails and absent (underdeveloped kneecaps) ABO blood type alleles linkage.
2/13= 0.154 recombination frequency
(this has been used to map Muscular Dystrophy gene to X chromosome
50% have mutation all have normal phenotype
Offspring will be:
We now know this deletion includes the locus that is within the region deleted for A+ but not for B+
a panel of deletions.
del a b c d e f
1 m + m + + m
2 m + + + + +
3 + m m m m +
4 + + m m m +
5 + + + m m +
6 + m + m + +
f?a f? c e d b
+ Human somatic cell
You set up a panel of these hybrid cells that include a random selection of human chromosomes
Look for which cell lines produce the product AND which chromosomes are present.
Bingo! You can determine which gene is associated with which chromosome!
More to come with our molecular discussions