Practical Hematology Lab. - LAB 10 -. Glucose 6 Phosphate Dehydrogenase G-6-P-D. Introduction. G6PD deficiency is an allelic abnormality which is inherited in an X-linked recessive fashion.
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- LAB 10 -
Glucose 6 Phosphate Dehydrogenase
When someone has G6PD deficiency, complications can arise; hemolytic anemia and prolonged neonatal jaundice are the two major pathologies associated with G6PD deficiency.
In G6PD deficient individuals, anemia is usually caused by certain oxidative drugs, infections, or fava beans.
NADP is reduced by G-6-PDH in the presence of G-6-P. The rate of formation of NADPH is directly proportional to the G-6-PDH activity and is measured spectrophotometrically as an increased in absorbance at 340nm.
If anemia and/or leukocytosis is present
Use Buffy coat free blood sample for assay (platelets and WBCs marked activity in this enzyme)
G-6-PDH (U/1012 RBC): (245-299)
G-6-PDH (U/g Hb): (6.97-20.5)