1 / 47

Charles L évesque , M.Sc . Product Manager- Analytical Chemistry Vincent B édard , B.Sc . HPLC Specialist François Bé

New Generic Approach for HPLC analysis: The Modern Silica-Based HPLC Columns; Silia Chrom ® Series. Charles L évesque , M.Sc . Product Manager- Analytical Chemistry Vincent B édard , B.Sc . HPLC Specialist François Béland, Ph.D . Vice-President of R&D. Outlines.

loc
Download Presentation

Charles L évesque , M.Sc . Product Manager- Analytical Chemistry Vincent B édard , B.Sc . HPLC Specialist François Bé

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. New Generic Approach for HPLC analysis: The Modern Silica-Based HPLC Columns; SiliaChrom®Series Charles Lévesque, M.Sc. Product Manager-AnalyticalChemistry Vincent Bédard, B.Sc. HPLC Specialist François Béland, Ph.D. Vice-President of R&D

  2. Outlines • HPLC Listing of all C18 • SiliaChrom Strategy • SiliaChrom—Chemistry Review • New Techologies-SB,AQ,XT • Evaluation of SiliaChrom • Recent SiliaChrom Applications • Generic Approach for HPLC applications • SiliaChrom Focus

  3. HPLC C18 column listing (L1-USP) • BioBasic 18 • Bio-Sil ODS • Bio-Sil ODS-10 • Bondclone C18 • BR-C18 • Burdick & Jackson C18 • Cadenza CD-C18 • Cadenza 5CD-C18 • Cadenza CL-C18 • Cadenza 5CL-C18 • Cadenza CW-C18 • Cadenza 5CW-C18 • Cadenza HS-C18 • Capcell C18 AG 120 • Capcell C18 SG 120, 300 • Capcell C18 UG 80, 120, 300 • Capcell C18 MG • Capcell C18 MGII • Capcell C18 MGIII • Capcell C18 ACR • Capcell C18 AQ • Capcell C18 IF • Chemcosorb 5-ODS-H • Chromegabond C18 • Chromegabond WR-C18 • Chromolith RP-18e • Clarity Oligo-RP • Clipeus C18 • Cogent HPS C18 • Cogent hQ C18 • Cogent Simulare C18 • Cogent MicroBee C18 • Cogent Bidentate C18 • Cogent e-Column C18 • Cosmosil C18 MS-II • Cosmosil C18 AR-II • Cosmosil C18 AR-300 • Cosmosil C18 PAQ • Alltima C18 • Alltima C18 LL • Alltima HP C18 • Alltima HP C18 EPS • Alltima HP C18 Hi-Load • Alltima HP C18 AQ • Alltima HP C18 Amide • Alltima WP C18 • AlphaBond C18 • Amethyst C18 • AminoQuant • APEX ODS • Apollo C18 • AQUA C18 • Aquapore OD-300 C18 • AQUASIL C18 • Ascentis C18 • Ascentis Express C18 • ASI C18 • ASI microC18 • Atlantis dC18 • Atlantis T3 • Axxi-Chrom C18, ODS • Bakerbond C18 • BetaBasic 18 • BetaMax Neutral • BETASIL C18 • Bio-C18 • Delta-Pak C18 • Denali • Develosil ODS • Diazem 2060 C18 60A • Diazem 300A C18 4-222 • Diazem 4-206 C18 • Diazem 4-230 C18 • Diazem 4-246 C18 • Diazem 4-247 C18EC • Diazem C18 • Diazem C18 1000 • Diazem C18 1120 • Diazem C18 120A • Diazem C18 120A series 43,44,45 and 46 • Diazem C18 series 200A, 300A • Diazem C18 series 2000 • Diazem C18 series 3000 • Diazem C18 series 4000 • Diazem C18 60A • ACE 300 C18 • ACE AQ • ACE C18 • ACE C18-AR • AccQTag Ultra • ACQUITY UPLC BEH130 • ACQUITY UPLC BEH 300 • ACQUITY UPLC BEH C18 • ACQUITY UPLC BEH Shield RP18 • ACQUITY UPLC HSS C18 • ACQUITY UPLC HSS C18 SB • ACQUITY UPLC HSS T3 • ACQUITY UPLC OST C18 • Adsorbosil C18 • Adsorbosphere C18 • Adsorbosphere HS C18 • Adsorbosphere UHS C18 • Adsorbosphere XL 300 C18 • Adsorbosphere XL C18 • Adsorbosphere XL C18-B • Allsphere ODS-1 • Allsphere ODS-2 • Allure C18 • Allure Aqueous C18

  4. HPLC C18 column listing (L1-USP) • Diazem C18 60A series 1,2,3 • Diazem C18 series EC • Diazem C18 Dual-Zone • Discovery C18 • Discovery HS C18 • Discovery Bo Wide Pore C18 • Econosil C18 • Econosphere C18 • Everest C18 • Fortis C18 • Fortis H2o • Gammabond C18 • Gemini C18 • Gemini-NX • Genesis C18 • GP-C18 • GraceSmart C18 • Haisil 100, 300 C18 • Haisil HL C18 • HALO C18 • Hi Chrom Reversible ODS • Hibar II C18 • Hibar ODS • HP-C18 • HxSil C18 • HydroBond PS-C18 • High Strength Silica (HSS) HPLC C18 • High Strength Silica (HSS) HPLC C18 SB • High Strength Silica (HSS) HPLC T3 • Hyperprep HS C18 • Hypersil 100 C18 • Hypersil BDS C18 • Hypersil GOLD • Hypersil GOLD aQ • Hypersil-ODS • Hypersil ODS-2 • Macrosphere 300 C18 • MetaSil AQ C18 • MetaSil ODS • MetroSep C18H • MetroSep C18AQ • MetroSep C18 AQ+ • MetroSep C18SH • MetroSep C18EPS • MPLC RP-18 • µBondapak C18 • µBondapak C18 Radial-Pak • Microsorb C18 • MicroSpher C18 • MonoChrom C18 • nanoAcquity BEH130 • nanoAcquity BEH300 • Nova-Pak C18 • NUCLEODUR C18 Gravity • NUCLEODUR C18 Isis • NUCLEODUR C18 Pyramid • NUCLEODUR 100 C18 ec • NUCLEODUR Sphinx RP • Nucleosil 100 C18 • Nucleosil C18 • Nucleosil C18 AB • Nucleosil C18 HD • Nucleosil C18 Nautilus • Nucleosil C18 MPN • Nucleosil C18 PAH • Nucleosil C18 PPN • ODS Ultrapak • OmniSpher C18 • Onyx C18 • Partisil ODS • Partisil ODS2 • Partisil ODS3 • Partisphere C18 HyPURITY AQUASTAR HyPURITY C18 IBM Octadecyl IB-SIL C18 IB-SIL C18 BD Inertsil ODS Inertsil ODS series 1,2,3 ISCO C-18 J’Sphere ODS-H80 J’Sphere ODS-L80 J’Sphere ODS-M80 Jupiter C18 Kinetex C18 Kromasil C18 LiChrospher PAH LiChrosorb RP-18 LiChrospher 100 RP-18 LiChrospher RP18e Luna C18 Luna C18(2) • Peco HCODS • Pecosil C18 • Pecosphere 3x8C • Pecosphere 3x8CR C18 • Pecosphere 5x15CRT C18 • Pecosphere-3C C18 • Pecosphere-3CR C18 • Pecosphere-3x3 C18 • Peptica C18 serresA,B,C • Pfarma C18 series A,B,C • Pinnacle II C18 • Pinnacle DB C18 • Pinnacle DB Aqueous C18 • Platinum C18 • Platinum EPS C18 • Platinum 300 C18 • Polaris C18-A • Polaris C18-Ether • Polygosil 60-7 C18 • Polygosil C18

  5. HPLC C18 column listing (L1-USP) • Polygosil C18 60ª • Premier C18 • Prep Nova-Pak HR C18 • Prevail C18 • Prevail OA • Prevail Select C18 • PrimeSphere C18-HC • Prodigy ODS(3) • ProntoSIL C18 H • ProntoSIL C18 SH • ProntoSIL C18 AQ • ProntoSIL C18 AQ Plus • ProntoSIL C18 ace-EPS • ProSphere 300 C18 • ProSphere 300 PAH • ProSphere HP C18 • ProSphere HP C18 AQ • ProSphere HP C18 ZAP! • ProteCol-G C18 HQ203 • ProteCol-P C18 HQ105 • ProteCol-P C18 HPH125 • ProteCol-P C18 GP125 • Purospher RP-18 • Purospher RP18e • Purospher STAR RP18e • Pursuit C18 • Pursuit XRs C18 • Radial-Pak C18 • Reduced Activity C18 • Regis ODS • Resolve C18 • Resolvex C18 • Sarasep P C18 • Scherzo SM-C18 • SelectaPore 90M, 300P, 300M • SynChropak RPP-1000 • SynChropak RPP-4000 • SynChropak RPP-100 • SynChropak RPS-100 • Synergi Fusion-RP • Synergi Hydro-RP • Targa C18 • TSK-GEL ODS-100V • TSK-GEL ODS-100Z • TSK-GEL ODS-100S • TSK-GEL Super-ODS • TSK-GEL ODS-80Tm • TSK-GEL ODS-80Ts • TSK-GEL ODS-120A • TSK-GEL ODS-120T • TSK-GEL ODS-140HTP • TSK-GEL OligoDNA-RP • Ultra C18 • Ultra Aqueous C18 • Ultra II C18 • Ultra II Aqueous C18 • Ultracarb ODS • Ultrasphere ODS • Ultrasphere C-18 • Ultrasphere C-18-IP • Ultron VX-ODS • Unison UK-C18 • Unison US-C18 • Velosep RP-18 • VisionHT C18 • VisionHT C18-B • VisionHT C18HL • VisionHT C18-P • Viva C18 • Vydac 201TP • Vydac 202TP • Vydac 218MS • Vydac 218TP • Vydac 238MS • Vydac C18 ShodexODSpak F-411 Shodex Silica C18M Shodex Silica C18P Spectraphysics C18 Spheri-(5 and 10) RP-18 Spheri-(5 and 10) ODS C18 Spherisorb ODS1 Spherisorb ODS2 Spherisorb ODS-B STR ODS-II STR ODS-M Sunfire C18 Supelcosil LC-18 Supelcosil LC-18-DB Supelcosil LC-318 Superiorex Superspher 100 RP-18 Symmetry C18 SymmetryPrep C18 Symmetry300 C18 SymmetryShield RP18 SynChropak RPP Wakosil C18 RS 3 and 5 Wakosil 5C18 HG and AR WakopakWakosil-II 3C18 HG WakopakWakosil-II 3C18 AR WakopakWakosil-II 3C18 RS WakopakWakosil-II 5C18 WakopakWakosil-II 5C18-100 WakopakWakosil-II 5C18 HG WakopakWakosil-II 5C18 AR WakopakWakosil-II 5C18 RS WakopakWakosil-II 5C18 HG Prep Wakopak Wakosil-II 5C18 AR Prep WakopakWakosil-II 5C18 RS Prep Wakopak Handy ODS WakopakWakosil 5C18 WakopakWakosil 5C18T WakopakWakosil 5C18N

  6. HPLC C18 column listing (L1-USP) YMC-Pack Hydrosphere C18 YMC-Pack ODS-A YMC-Pack ODS-AL YMC-Pack ODS-AM YMC-Pack ODS-AQ YMC-Pack Pro C18 YMC-Pack Pro C18 RS Zodiac 120 C18H Zodiac 120 C18AQ Zodiac 120 C18 Eurobond Zodiac 120 AC EPS Zodiac 120 C18SH Zodiac ODS Zodiac ODS H ZORBAX ODS C18 ZORBAX ODS Classic ZORBAX Eclipse Plus C18 ZORBAX Eclipse PAH ZORBAX Eclipse XDB-C18 ZORBAX Extend-C18 ZORBAX Rx C18 ZORBAX StableBond C18 ZORBAX 300 StableBond C18 • WakopakWakosil 10C18 • WakopakWakosil 5C18 AR • WakopakWakosil 5C18-200 • WakopakWakosil 5C18-200T • WakopakWakosil 5C18-200N • WakopakWakosil 10C18-200 • Wakopak MS-5C18 GT • WakopakNavi C18-5 • WakopakCombi ODS • WakopakCombi ODS Fast • XBridge BEH130 • XBridge BEH300 • XBridge C18 • XBridge OST C18 • XBridge Shield RP18 • XTerra MS C18 • XTerra RP18 • 378 HPLC column as C18 in 2010 • Which is the best one! • Do I need to buy all! • What is my C18 strategy? • ...etc... $$$

  7. A new modern strategy is absolutely necessary in 2010 for HPLC end-users New Generic Approach to Find Appropriate HPLC column (Total Solution for HPLC end-users) Better matrix (silica-gel) -Stationary Phase Specification (manufacturers) Phase according to Polarity (acidic and basic analytes) Phase according to Hydrophobicity(% carbon loading) Phase according to Low Metal Content and Silianol Activity Phase compatible with new LC-MS technology Phase for very aqueous mobile phase (100% buffer or water) Etc... Note: Every Pittcon and Analytica Events present new C18, more and more HPLC C18/year

  8. New Modern HPLC Strategy • How to improvetodaysilica-based HPLC columns? • Betterbondingchemistry/ improved control for betterreproducibility. • What about the matrix? (support—silica) • High QualityLevel of the Support (Silica) • narrowest particule size distribution • perfectlyspherical • highlevel of purity • High surface area (m2/g) • Efficiency pore size (Å) • No fines SiliaSphere (SiliCycle) • Lowmetal content • High mechanicalstability • Reproducibleloading (critical in reversed-phase) X X Supplier A (top) Supplier B (bottom) (both are real HPLC supports)

  9. GenericApproach/Most popular applications • Nowbetter support (Silica) • Similarchemistriesthanother HPLC suppliers • Improvedquality and efficiency • HPLC column forlow pH (not hybrid, pure silicabased) • HPLC column forhigh pH (pseudo-hybrid) • HPLC column for 100% aqueous mobile phase (or buffer) • HPLC column for LC-MS (no bleeding) • HPLC column for QC/QA • validation and method development (easy method) • No tailling, bettersensitivity and peakshape for quaternary ammonium compounds) • HPLC column for HILIC technology(extremely polar compounds)

  10. Portofolio of SiliaChromColumns A column for everyneed: ONLY 7 Chemistries! SiliaChromSB C18 and SB C8 SiliaChrom XDB1 C18 ,C8, C4, CN, AMINO, PHENYL, DIOL, Silica… SiliaChromXDB and XDB2 (C8&C18) SiliaChrom AQ C18 (dtC18) SiliaChromHILIC SiliaChrom XT C18 (XT Fidelity C18) NO MORE CHEMISTRIES

  11. SiliaChrom-Chemistries Review New Technology Silica-Based HPLC columns for Extremely Acidic Compounds R=C8 or C18, pH<2.0 H3O+ Steric Effect H3O+ Note: Not Endcapped silica gel

  12. SiliaChrom-Chemistries Review New Technology TMS endcapped Silica-Based HPLC columns for all type of compounds (pH=2-9) Free silianol groups (OH) R=C18 Polar Surface Ratio of TMS-OH-C18 is well-balanced Note: Partially End capped silica gel

  13. SiliaChrom-Chemistries Review Silica-Based HPLC column for EXTREMELY POLAR compounds SiliaChromHILIC Normal-Reversed Phases Conditions: Only SiliaChromHILIC Note: Not Endcapped silica gel

  14. SiliaChrom-Chemistries Review Silica-based HPLC columns for low to high hydrophobic compounds pH=1.5-10.0 SiliaChromXDB C8 (8%) and C18 (15%) SiliaChrom XDB1 C8 (14%) and C18 (22%) SiliaChrom XDB2 C18 (18%) 1) Different carbon loadings (8-22%C) 2) XDB2=Luna C18(2) 3) XDB1= Phenyl, NH2, CN, 300A, etc… Note: Double Endcapped silica gel

  15. SiliaChrom-Chemistries Review New Technology Replace OH by CH3 (Pseudo-Hybrid tech.) SiliaChromXT C18 Series Silica-Based HPLC columns for EXTREMELY Basic compounds pH=1.5-12 No bridge (No Backpressure issue as XBridge (Waters))

  16. SiliaChrom-Chemistries Review

  17. SiliaChromSeriesHPLC Classification-USP/NISTApproaches • All SiliaChrom HPLC columns are evaluated by USP and NIST approaches: • Uracil: void volume marker • Toluene: hydrophobicretention • Ethylbenzene: methyleneselectivity and plate count determination • Amitriptyline: activitytowards bases (silianolactivityevaluation) (A) • Quinizarin: activitytowardschelatingreagents (metal content evaluation) (B) (Pharmacopeial Forum, Vol. 31(2) March-Apr. 2005, p.637) (NIST, SRM 870, Certificat Issue Date:December 19th 2002, ValiduntilSeptember 30th 2010) (A) (B)

  18. SiliaChromEvaluation • HPLC System: Thermo Surveyor with PDA • (ChromQuest-Data handling version 5.0) • Mobile Phase: MeOH/buffer (80/20, v/v) • Buffer: 20mM of phosphate buffer adjusted at pH=7.0 • Flow rate: 1.0 mL/min • Detector: UV scan (PDA), Total scan 200-600nm • Temperature: 23C • All HPLC columns: 4.6x150mm, 5µm

  19. 150000 140000 130000 120000 110000 1.55 100000 90000 7.34 80000 uAU 9.73 70000 60000 3.88 4.86 50000 40000 30000 20000 10000 0 -10000 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Time (min) Phenomenex Luna C18(2) Low Plate Count (seeResults Table) Uracil-Toluene-Ethylbenzene-Quinizarin-Amitriptyline

  20. 180000 170000 160000 150000 140000 1.38 130000 120000 110000 100000 90000 6.85 uAU 80000 70000 3.29 60000 4.16 50000 12.99 40000 30000 20000 10000 4.73 0 -10000 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Time (min) Agilent ZorbaxSB C18 Important Tailling Uracil-Toluene-Ethylbenzene-Quinizarin-Amitriptyline

  21. 150000 140000 130000 120000 1.49 110000 100000 90000 80000 uAU 70000 10.18 60000 8.71 4.05 50000 5.14 40000 30000 20000 10000 0 -10000 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Time (min) Waters SunfireC18 Asymetry Metal Contamination Uracil-Toluene-Ethylbenzene-Quinizarin-Amitriptyline

  22. 190000 180000 170000 160000 150000 1.61 140000 130000 120000 110000 100000 uAU 90000 80000 8.75 70000 12.02 60000 4.15 5.19 50000 40000 30000 20000 10000 0 -10000 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Time (min) SiliaChrom XDB2 C18 Very good peak shapes Uracil-Toluene-Ethylbenzene-Quinizarin-Amitriptyline

  23. Summary Results-Case Study Basic analyte: SiliaChrom XDB2 Less polar analyte: SiliaChromXDB1

  24. Recent SiliaChromApplications • Acidic and Basic Compounds (8) with one SiliaChrom HPLC column (AQ C18) • Fluoroquinolones for Veterinary use by HPLC-Marbofloxacin and Sarafloxacin • Halogenated Herbicides by HPLC-Dicamba, Atrazine and 2,4-D • Catecholamines by HPLC- Polar Compounds • Faster HPLC withSiliaChrom-Polar and mid-polar Compounds • Isomerseparation for Chlorophenolseries by simple HPLC method • HPLC study for Naloxone-pH=2.5, 7.0 and 10.5 • HPLC Chromatogram of Ganciclovir– Polar Compound (diol-amine) • HPLC Chromtogram of Cefotaxime—Extremely Polar Compound (acid-amine-sulfur) • And more….

  25. Recent SiliaChromApplications Acidic and Basic Compounds (8) withsameSiliaChrom HPLC column 1-MaleicAcid 4-Chlorpheniramine 7-Dichlorphen 2-Acetaminophen 5-BenzoicAcid 3-Pseudoephedrine 8-Ibuprofen 6-SalicylicAcid

  26. Recent SiliaChromApplications Acidic and Basic Compounds (8) withsameSiliaChrom HPLC column Phenomenex Luna C18(2) 5µm 4.6x150mm 210 nm 1 2 2 5 2 230 nm 230 nm 4 6 7 1 3 8 1

  27. Recent SiliaChromApplications Acidic and Basic Compounds (8) withsameSiliaChrom HPLC column (same HPLC conditions than Luna C18(2)) SiliaChrom AQ C18 5µm 4.6x150mm 210 nm 2 5 230 nm 4 6 7 3 8 1

  28. Recent SiliaChromApplications Fluoroquinolones for Veterinary use by HPLC-Marbofloxacin and Sarafloxacin SiliaChrom AQ C18 5µm 4.6x150mm Zwitterion Molecules Waters Atlantis dC18 5µm 4.6x150mm Note: SiliCycle has the perfect equivalent (better) with SiliaChromAQ C18 (or dtC18) than Atlantis dC18 (Waters)

  29. Recent SiliaChromApplications Halogenated Herbicides by HPLC-Dicamba, Atrazine and 2,4-D SiliaChrom dtC18 5µm 4.6x150mm

  30. Recent SiliaChromApplications Halogenated Herbicides by HPLC-Dicamba, Atrazine and 2,4-D SupelcoAscentis C18 5µm 4.6x150mm

  31. Recent SiliaChromApplications Catecholamines by HPLC-Polar Compounds SiliaChrom AQ C18 5µm 4.6x150mm Mobile Phase: Water 1.0% AcOH only! EpinephrineNorepinephrine Dopamine Tyrosine Levodopa

  32. Recent SiliaChromApplications Faster HPLC withSiliaChrom-Polar and mid-polar Compounds SiliaChrom AQ C18 5µm 3.0x50mm 95/5 0.1%TFA in H2O/ACN – 5/95 0.1% TFA in H2O/ACN in 2.25 min. 1.75 mL/min, 23°C, 254 nm (LC-MS conditions fromAstraZeneca Canada) Test Mix to evaluate HPLC column from AstraZeneca (Canada) CaffeineChlorthalidoneAlbendazoleWarfarinTolnaftateDioctylPhatalate

  33. Recent SiliaChromApplications SiliaChrom XDB1 PHENYL 5µm 4.6x150mm 60/40, H2O/MeOH, 1.00 mL/min, 23°C, 254nm : EasyMethod!

  34. Recent SiliaChromApplications • HPLC study for Naloxone-pH=2.5, 7.0 and 10.5 SiliaChrom XT C18, 5µm, 4.6x150mm 80/20, Phosphate Buffer/MeOH, 1.00 mL/min, 23°C, 254nm Fronting ? pH=2.5 Assymetry = 0.85 50/50, Phosphate Buffer/MeOH, 1.00 mL/min, 23°C, 254nm pH=7.0 Assymetry = 0.89

  35. Recent SiliaChromApplications • HPLC study for Naloxone-pH=2.5, 7.0 and 10.5 SiliaChrom XT C18, 5µm, 4.6x150mm 50/50, H2O/MeOH 0.1% NH4,OH, 1.00 mL/min, 23°C, 254nm pH=10.5 Assymetry = 0.99 Remember, pH range for XT is 1.5-12—Good Chromatography Tool

  36. Recent SiliaChromApplications HPLC Chromatogram Ganciclovir – Polar Compounds (diol-amine) SiliaChrom AQ C18 (4.6x150mm, 5µm) MeOH:Water, 5:95 UV 252nm 1mL/min

  37. Recent SiliaChromApplications HPLC Chromatogram of Cefotaxime—Extremely Polar compound (acid-amine-sulfur) SiliaChrom AQ C18 (4.6x150mm, 5µm) 0.4 mM KH2PO4 + 8mM K2HPO4: MeOH, 89:11 UV 254nm 1mL/min

  38. Recent SiliaChromApplications HPLC for mixture of Fenitrothion-Pyriproxyfen—Different Level of Hydrophobicity SiliaChrom XDB1 C18 (4.6x150mm, 5µm) ACN:Water, 70:30 UV 254nm 1mL/min

  39. Recent SiliaChromApplications HPLC Determination for Ephedrine (from Ephedra Sample) Zorbax SB C18 (4.6x150mm, 5µm) SiliaChrom XDB2 C18 (4.6x150mm, 5µm)

  40. Recent SiliaChromApplications HPLC chromatogram of Berberine-Metal Content Effect SunfireC18 (4.6x250mm 5µm) SiliaChromdtC18 (4.6x250mm, 5µm) ACN: 1%TFA, 35:65 UV 346nm 1 mL/min

  41. Durability Testing at pH-1.00 SiliaChrom SB C18 0 hr 120 hrs SiliaChromSB C18 (4.6x150mm, 5µm) 59:39:2 ACN:Water:TFA (pH = 1.00) UV 270 nm, 1 mL/min (Uracil, Phenol, Toluene and Ethylbenzene) • Poster presented at Pittcon 2010, Orlando FL, USA • LC-GC Application Note—September 2009

  42. Durability Testing at pH-11.5 SiliaChromXT C18 Naphthalene 2.75 2.25 1 hr 1.91 2.23 2.77 SiliaChromXT C18 (4.6x150mm, 5µm) MeOH: Water/TEA, pH=11.5, 40:60 UV 215nm, 1 mL/min 1.84 400 hrs Uracil, Phenol, Nitrobenzene and Naphtalene

  43. Durability Testing at pH=11.50, 90C SiliaChrom XT C18 Fidelity 0 hr SiliaChromXT C18 (4.6x150mm, 5µm) 54.9-44.9-0.2 (ACN-Water-TEA) 90C, UV 270 nm 1 mL/min 120 hrs (Uracil, Phenol, Toluene and Ethylbenzene)

  44. SiliaChrom HILIC, Vitamins SiliaChrom HILIC (4.6x150mm, 5µm) ACN 0.1% TFA: Water 0.1%TFA, 10:90 UV 280nm 1 mL/min Waters HILIC Silica HPLC column (4.6x150mm, 5µm) B6 C B2 B1 Vitamin B1, Vitamin B6, Vitamin C and Vitamin B2

  45. Generic Approach with SiliaChrom Series 87% of HPLC success in my Lab! Neutral Note: SiliaChrom AQ is an universal HPLC column (see how particle is designed : TMS-OH-C18)

  46. Conclusion ONLY 8 Chemistries to cover more than 85% of Regular HPLC Application using Silica-Based as support • Be aware for your HPLC column strategy, Marketing is not real SCIENCE!

  47. www.silicycle.com Thank you!

More Related