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Identification Guide for High Mass Compounds

This flow chart provides guidance for identifying high-mass compounds above 700 Da, requiring separation, containing one or two polar functional groups, with limited functionality, and insoluble characteristics. It helps determine the solubility, presence of polar groups, separation needs, and mass spectrometry methods. The guide is particularly useful for identifying polymers, peptides, proteins, and compounds with varied functionality.

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Identification Guide for High Mass Compounds

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  1. Is your mass above 700 Da? N Y Y GCEI Do you require separation of a mixture? Y Is it a polymer? Y Does it have limited functionality? MALDI N EI N N N Does it contain only one or two polar functional groups? Y EI or CI Is it soluble and contain a number of polar groups? N Y Y Y LSIMS or DART Is it Insoluble? Is it Cationic/Anionic? Do you require separation of a mixture? Is it a peptide or protein? N N N Does it contain a large number of polar groups? Y ES Y Are there buffers or detergents present? N Y ES Will it elute under normal reverse phase conditions? Y N MALDI Y LCES UPC2 Please note: Flow Chart is not exhaustive and should be used as a guide only.

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