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This study compares benthic invertebrate populations in shallow and deep water areas of Ingle's Pond, analyzing sediment type, organic material, and species diversity. Results show differences in taxa distribution and organic content. Conclusions highlight implications for fish populations. Acknowledgments, references, and data analysis methods are detailed.
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Benthic Analysis: Mr. Ingle’s Pond X Group Tarah Johnson McClure Tosch Stephen Wells Lance Keller
Overview • Objectives • Study Area • Materials & Methods • Results • Conclusions • Summary
Objectives Shallow Water vs. Deep Water • Sediment Type • Organic material • Benthic invertebrate population
Study Area • Deep: 9’2” • Shallow: 3’ 5”
Methods • Assessment was split into two parts: • Benthic sampling for macro invertebrates • Eckman Dredge • 15 cm x 15 cm • Weight sampling for sediment loading and organic concentration • KB corer • 2 inches in diameter
Methods • Both assessments were taken in triplicate totaling 6 samples each • Deep • 3 Eckman Dredge samples • 3 Core samples • Shallow • 3 Eckman Dredge samples • 3 Core samples
Methods: Eckman Samples • Sieved on site • Biota preserved with formalin • Transported to lab for analysis
Methods: Eckman Samples • Filtered to wash off formalin • 80 micrometer (μm) sieve • Large samples • Sub-sampled when needed • Observed under dissecting scope • Counted and identified to family • T-test and an ANOVA • Difference in count and composition
Methods: Core Samples • Core samples were sectioned on site • 2cm sub-sample to 10cm • Wet weight • Crucible weight (zeroed out) • Crucible + sediment sample
Methods: Core Samples • Oven Dry Weight • 24 hours at 150° C • Organic Weight • Furnace: 3 hours at 500° C • T-test and an ANOVA • difference in composition between shallow and deep samples.
Greater Diversity in shallow samples Shallow samples were more even distributed Shallow samples had more taxa Results: Eckman Samples
Results: Eckman Samples • Major Taxa Found - Chaoboridae - Chironomus • Minor Taxa Found - Ceratopogonidae - Oligochaeta
Results: Core Samples • There was no difference in moisture content • There was a difference in percent organic • The sediment is mainly composed of silt
Conclusions • Fish feed on Chaoboridae and Chironomus larvae (Sweetman 2006). • Chironomus larvae suggest highly eutrophic pond (Saether 1979) • Chaoboridae larvae specifically Chaoborus migrate vertically to avoid predation (Sweetman 2006).
Conclusions • The percent water content suggests a silt/clay sediment • Dr. McDaniel concluded the sediment was mostly silt • The difference in percent organic might be from differences in Chaoboridae numbers.
Summary • The high Chaoboridae and Chironomus populations are good fish food but might not be available to eat. • There is a difference in organic soil content between deep and shallow probably due to Chaoboridae pop. • There was no difference in sediment loads between the shallow and deep end of the lake.
Acknowledgments • Mr. Ingle • Picture Credits • iodeweb5.vliz.be/.../AndersonBook/SampEquip.htm • Dr. Wilhelm • Other Credits • X Group Bathometric Group • X Group Light and Temperature Profiles
References • Jon N. Sweetman, a, and John P. SmolaReconstructing fish populations using Chaoborus (Diptera: Chaoboridae) remains – a review Paleoecological Environmental Assessment and Research Laboratory (PEARL), Department of Biology, Queen's University, Kingston, Ont., Canada K7L 3N6 • Saether, O.A. 1979. Chironomid communities as water quality indicators. – Holarct Ecol. 2: 65-74