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PCR-Based DNA Fingerprinting for Identification and Paternity Testing

Learn about PCR-based DNA fingerprinting, a powerful technique to identify criminals, determine paternity, and identify human remains. Discover how polymorphic DNA regions serve as markers for disease and how AmpliFLPs and VNTR analysis are used in DNA profiling.

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PCR-Based DNA Fingerprinting for Identification and Paternity Testing

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  1. PCR A license to do molecular biology

  2. DNA FINGERPRINTING BY PCR

  3. POLYMORPHIC DNA • Chromosomal regions that vary widely from individual to individual • By examining several of these polymorphic regions within the DNA, one may obtain a “DNA fingerprint”

  4. Can identify criminals • Can determine paternity • Can identify human remains • Serve as markers for certaindiseases

  5. Most commonly used polymorphisms are those which vary in length • FLPS-fragment length polymorphism • When amplified by PCR referred to as AmpliFLPs

  6. VNTR • Variable number of tandem repeats • FLPs that contain specific sequences duplicated a variable # of times

  7. D1S80 • Is a specific type of VNTR • Located on chromosome 1 • Contains a 16 nucleotide sequence • Variable repeated 16-40 times • D1S80 locus is one VNTR used by FBI

  8. An individual who is homozygous For the D1S80 genotype will have equal repeat numbers on both homologues of chromosome 1 Yields a single PCR product seen on gel An individual who is heterozygous for the D1S80 genotype will have different D1S80 repeat numbers Yields 2 distinct PCR products seen on gel

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