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The MRSEC Microfluidics Shared Facility Seth Fraden , Dongshin Kim Brandeis University MRSEC DMR-0820492. Collaborations with Facility Users.

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The MRSEC Microfluidics Shared FacilitySeth Fraden, Dongshin Kim

Brandeis University MRSECDMR-0820492

Collaborations with Facility Users

During the academic year, Fall09 – Spring10, Dr. Kim, the facility director ,designed, built and tested devices for a number of MRSEC and outside users.(a)Microfluidic Device to Study of the Structure of Chromosomes (Dr. Wiggins). This is part of the “confined polymers” thrust. The origin of replication of the E-coli chromosomes is labeled with a parB-GFP fluorescent fusion protein to track the locus dynamics in live cells during chromosome replication. The cells are loaded using a permeation pump designed into the microfluidic device which is made from agarose gel so that drugs and growth media can be infused into the cells. (b) Top view of a Microfluidic oxygenation device for brain tissue culture (Prof. Lisman) to study neurons. Gas and fluid channels are separated by a thin (5 micron) PDMS membrane.

(a)

10 mm

(b)

1 mm