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Biochemistry I. Labs 22,23,24. Exoenzymes – Lab 22. Bacteria make enzymes to digest macromolecules outside of the cell The smaller sub units of the enzymatic reaction are then able to be transported across the cell membrane This capacity is a characteristic of many Gram Positive bacteria.

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biochemistry i

Biochemistry I

Labs 22,23,24

exoenzymes lab 22
Exoenzymes – Lab 22
  • Bacteria make enzymes to digest macromolecules outside of the cell
  • The smaller sub units of the enzymatic reaction are then able to be transported across the cell membrane
  • This capacity is a characteristic of many Gram Positive bacteria
hydrolysis of starch
Hydrolysis of starch
  • Starch is composed of glucose molecules connected by glycosidic linkages
  • When the glycosidic linkages are broken, individual sugar molecules are produced
  • Linkages are broken in the presence of Water and the enzyme amylase
hydrolysis of gelatin
Hydrolysis of Gelatin
  • When water and an enzyme are added to a protein, the peptide bond in proteins are broken, producing individual amino acids
fermentation
Fermentation
  • Phenol red – pH indicator is red in a neutral environment and yellow is the result of an acidic pH
  • Gas – Gas is a by -product of the fermentation reaction -
triple sugar iron
Triple Sugar Iron
  • a. metabolize lactose and/or sucroseb. conduct fermentation to produce acidc. produce gas during fermentationd. generate H2S.