slide1 l.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract PowerPoint Presentation
Download Presentation
The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract

Loading in 2 Seconds...

play fullscreen
1 / 14

The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract - PowerPoint PPT Presentation


  • 181 Views
  • Uploaded on

The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract . by Niel Henriksen Mentor: Dr. Phil Proteau College of Pharmacy. Hawthorn and Heart Failure.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide1

The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract

by Niel Henriksen

Mentor: Dr. Phil Proteau College of Pharmacy

hawthorn and heart failure
Hawthorn and Heart Failure
  • Congestive heart failure (CHF) results from coronary artery disease, a previous heart attack, high blood pressure, heart defects, and infections in the heart tissue.
  • Nearly 5 million people in the United States are diagnosed with CHF.
  • The symptoms of CHF include shortness of breath, fatigue, swelling and kidney dysfunction.
hawthorn extract benefits
Hawthorn Extract Benefits
  • Clinical trials by the NYHA demonstrated:

(+) exercise tolerance

(-) dyspnea (shortness of breath)

(-) ankle swelling

(-) blood pressure

  • Studies on cardiac tissue show:

(+) inotropic effects

(+) cardiac flux

(+) vasorelaxation

(+) anti-arrhythmic potential

hawthorn extract unknowns
Hawthorn Extract Unknowns
  • Active compound(s)
    • Flavonoids and Procyanidins?
  • Mechanism of action
  • Proper dosing
  • Duration of therapy
  • Most effective extract source (berries, leaves, or flowers)
project goals
Project Goals
  • Develop cell based bioassays for evaluation of the direct cardiac effects of hawthorn extract
  • Using bioassay-guided fractionation, identify the components in hawthorn extract with cardiac activity
bioassay tests
Bioassay Tests
  • Rate of Contraction
  • Amplitude of Contraction
isolation techniques
Isolation Techniques
  • Solvent Extraction
  • Thin-Layer Chromatography
  • Large Scale Chromatography
  • High Performance Liquid Chromatography
identification techniques
Identification Techniques
  • Mass Spectrometry
  • Nuclear Magnetic Resonance (NMR) Spectroscopy
future goals
Future Goals
  • Complete bioassay-guided fractionation
  • Determine the most effective extract source
  • Determine the mechanism through with hawthorn extract functions
  • Correlate the plasma levels of cardioactive component with the physiologic effects on animal models
acknowledgements
Acknowledgements
  • Dr. Phil Proteau
  • Shannon Long
  • Dr. Theresa Filtz
  • Dr. Kevin Ahern
  • Howard Hughes Medical Institute