Rhizome Project Meeting(1) 5-4-2011. Min- Jeong Kim Gang Lab Institute of Biological Chemistry Washington State University Pullman, WA. Approach. gene A. gene B. mRNA. microarray. RNA- seq. Intensity of spot. Number of sequence. Expression level. Work Flow. Flow cell.
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Institute of Biological Chemistry
Washington State University
Intensity of spot
Number of sequence
Qiagen RNA mini kit
Attach DNA to flow cell surface
Prepare DNA sample
Fragments become double stranded
Sequencing by synthesis(SBS)
* Some are not available in the database
Adding the sequence index to a library
Pooling 5 replicates in a tube