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Antigen-Antibody Interactions. Dr. Conrad Sept. 10,2008. Antigenicity. Requirements to be an immunogen Low mw antigens (haptens) Adjuvants Primary vs secondary responses. Concept of a Hapten. r/c = Kn-Kr. c = concentration of free antigen (hapten) K = K a = equilibrium constant

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antigen antibody interactions

Antigen-Antibody Interactions

Dr. Conrad

Sept. 10,2008

antigenicity
Antigenicity
  • Requirements to be an immunogen
  • Low mw antigens (haptens)
  • Adjuvants
  • Primary vs secondary responses
slide8

r/c = Kn-Kr

c = concentration of free antigen (hapten)

K = Ka = equilibrium constant

r = hapten bound per unit Ab

n = Valence of Ab

factors which influence ab affinity
Factors which influence Ab affinity
  • Temp, pH, ionic strength
  • Polyclonal vs monoclonal
  • Ab class
  • Type of antigen
slide23

Comparison of Hv sequences of monoclonals binding the

Same vs binding a different antigen epitope

slide26

Affinity vs avidity – depends on valence

Avidity – sum total or strength of binding of two molecules (or cells) to one another at multiple sites. Distinct from affinity which is the strength of binding of one site on a molecule to its ligand.

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