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Advanced Chromatographic Techniques for the Study of Thyme Chemotypes and Polyphenols

Explore the analysis of 7 thyme chemotypes rich in linalool, carvacrol, thymol, and more. Learn about the isolation of polyphenols like coumarins and anthocyanins, enabled by Oplc semi-preparative mode. Enhance your research with rapid scalability and compound collection abilities. Personal works include isolation of various compounds from hemp, opium, and more.

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Advanced Chromatographic Techniques for the Study of Thyme Chemotypes and Polyphenols

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Presentation Transcript

  1. Study of 7chemotypes of thymes Thymes: Linalool,thuyanol,carvacrol, thymol, lemony,wild,geraniol, borneol.

  2. POLYPHENOLS

  3. Coumarins • Aesculus hippocastanum Ethyl acetate /chloroform 60/40 v/v on silicagel

  4. Flavonoids Ethyl acetate /chloroform 40:60 v/v on silicagel

  5. Anthocyanins • Malva sylvestris Ethyl acetate MEK formic acid hydrochloric acid 65/10/6/9 v/v on silicagel

  6. Oplc semi preparative mode • ability to perform high performance separations of difficult samples with little to no sample preparation • ability to visually check directly on the chromatographic phase that all compounds have actually been eluted • abiity for rapid scale-up on the same instrument • ability collect several tenth of mg of purecompounds • ability to select either on-line or off-line injection depending on the sample matrix

  7. Oplc semipreparative mode 2 • Personal works • isolation of • cannabidiol from hemp • strychnine, brucine from strychnos • lupanine from lupinus • morphine, codeine, thebaine, papaverine, noscapine from opium

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